基于形态学和EF-1α序列特征的我国高粱子粒寄藏镰孢菌种群鉴定  

Identification of Sorghum Seed-Borne Fusarium Population Based on Fungi Morphology and EF-1α Sequence

作　　者：胡兰[1] 姜钰[1] 徐秀德[1] 

机构地区：[1]辽宁省农业科学院植物保护研究所,辽宁沈阳110161

出　　处：《作物杂志》2013年第4期129-132,共4页Crops

基　　金：现代农业产业技术体系建设专项(CARS-06-02-01)

摘　　要：采用形态学和EF-1α基因序列分析相结合的方法,对我国13个省17个市区的274份高粱子粒样品中分离获得的313株镰孢菌进行鉴定。在313株镰孢菌中,共鉴定出13个镰孢菌种:产黄色镰孢(Fusarium thapsinum)、禾谷镰孢(F.graminearum)、层出镰孢(F.proliferatum)、变红镰孢(F.incarnatum)、藤仓镰孢(F.fujikuroi)、轮枝镰孢(F.verticillioides)、亚粘团镰孢(F.subglutinans)、木贼镰孢(F.equiseti)、茄镰孢(F.solani)、尖孢镰孢(F.oxysporum)、九州镰孢(F.kyushuense),尚无中文名称的F.miscanthi和F.lacertarum。产黄色镰孢(F.thapsinum)、禾谷镰孢(F.graminearum)、层出镰孢(F.proliferatum)和变红镰孢(F.incarnatum)是高粱子粒寄藏镰孢菌种群中的优势种类。从分离频率和地域分布广泛性来看,我国高粱产区高粱子粒上均可分离获得镰孢菌,河南、山东、四川的分离频率最高,分别达43.87%、43.73%和40.44%。Three hundred and thirteen Fusarium isolates from 274 samples of sorghum grains in 17 cities of 13 provinces were identified by fungi morphology and EF-1α sequence analysis. Among 313 isolates of Fusarium, a total of 13 Fusarium species was founded, including Fusarium thapsinum, F. graminearum, F. proliferatum, F. incarna- turn, F. fujikuroi , F. verticillioides , F. subglutinans , F. equiseti , F. solani , F. oxysporum , F. kyushuense , F. miscanthi , and F. lacertarum. F. thapsinum , F. graminearum , F. proliferatum , and F. incarnatum were dominant Fusarium spe- cies. The sorghum grain samples from all sampling sites were contaminated with Fusarium spp.. The grain from Henan had the highest isolation frequency（43.87% ）of Fusarium spp. ,followed by Shandong（43.73% ） and Si- chuan province （40.44%）.

关 键 词：高粱 子粒寄藏真菌 分离频率 镰孢菌 

分 类 号：S435.14[农业科学—农业昆虫与害虫防治]