贵州3个地域苗族人群mtDNA 9bp序列缺失频率  

Analysis on frequency of 9 bp deletion of mitochondrial DNA in Miao population in three geographical regions of Guizhou

作　　者：任凌雁[1] 何燕[1] 张婷[1] 王婵娟[1] 王欢[1] 官志忠[1] 单可人[1] 

机构地区：[1]贵阳医学院分子生物学重点实验室,贵阳550004

出　　处：《重庆医学》2013年第22期2586-2587,2590,共3页Chongqing medicine

基　　金：贵州省科技厅社会发展攻关项目[黔科合SY(2010)3001号];贵州省科技厅基金项目[黔科合J字(2011)2119号];贵州省科技厅基金项目[黔科合SY(2012)3135号]

摘　　要：目的分析贵州3个地域苗族人群线粒体DNA COⅡ/t RNALys基因间小非编码V区串联重复序列9bp缺失频率。方法采用聚合酶链反应-聚丙烯酰胺凝胶法(PCR-PAGE)及DNA序列分析法进行检测。结果从江岜沙、黔西沙井、威宁龙街等3个地域苗族人群缺失频率分别为19.15%(9/47)、42.5%(17/40)和29.55%(13/43);岜沙苗族人群与沙井苗族人群9bp缺失频率比较差异有统计学意义(P<0.05)。结论 3个地域苗族人群mtDNA 9bp缺失基因频率较高,不同地域苗族人群间缺失频率有一定差异。Objective To analysis the frequency of 9bp deletion polymorphism of mitochondrial DNA（mt DNA）among Miao population in three geographical regions of Guizhou.Methods Polymerase chain reaction and polyacrylamide gel electrophoresis（PCR-PAGE）were used in the study of mtDNA 9bp deletion polymorphism,DNA sequence was used to verify the results.Results Each group who come from Basa,Shajin,Longjie in Guizhou,the frequency of 9bp deletion of mtDNA value as 19.15%（9/47）,42.5%（17/40）and 29.55%（13/43）.Short polymorphic frequency difference was statistically significant between Basa and Shajin groups（P0.05）.Conclusion The frequencies of 9bp deletion of mtDNA are relatively high in Miao ethnic group in Guizhou.There are certain differences of the delegation frequencies among these groups.

关 键 词：线粒体DNA 遗传多态性 苗族 贵州 

分 类 号：Q987[生物学—遗传学]