两种不同检测法对猪瘟抗体阴性猪筛检的比较  

Comparison of two different methods for screening swine fever antibody-negative pig

作　　者：李小静[1] 唐满华[1] 邓月嫦 叶健聪 

机构地区：[1]广东大华农动物保健品股份有限公司,广东云浮527400

出　　处：《广东畜牧兽医科技》2013年第4期37-38,共2页Guangdong Journal of Animal and Veterinary Science

摘　　要：猪瘟抗体阴性猪是猪瘟活疫苗安检的重要实验动物,目前对于猪瘟抗体阴性猪筛选是采用兔体中和反应法,实验复杂,工作量大并且易受实验兔个体差异等因素影响。随着生物学及其技术的发展,ELISA被广泛用于生物制品的各个领域。本试验应用ELISA法和兔体中和反应法对猪瘟抗体阴性猪进行筛选,共检测160份血清,检测结果显示两种方法结果有很高的一致性,但ELISA法更为敏感,快捷,更适用于猪瘟抗体阴性猪的筛选。Swine fever antibody-negative pig is important laboratory animals for security inspection of swine fever vaccine. At present, rabbit neutralization test is used for screening swine fever antibody-negative pig, but the experiment is complex,laborious and is influenced by many factors such as individual differences of rabbits. With the development of biotechology, ELISA is widely used in various fields of biological products. In this paper, compared with rabbit neutralization test,ELISA was used for screening swine fever antibody-negative pigs from 160 serum samples. The result showed that the two methods had good consistency, but the method of ELISA was more sensitive and fast,being suitable for screening swine fever antibody negative pigs.

关 键 词：ELISA法 兔体中和反应法 猪瘟抗体阴性猪 

分 类 号：S852.65[农业科学—基础兽医学]