不同时间给予大红袍水提物对小鼠急性酒精性肝损伤的影响  

Influence of Dahongpao Tea Water Extract Feeding at Different Time on Alcohol-induced Acute Liver Injury in Mice

作　　者：曹丹[1,2] 刘仲华[1,2] 黄建安[1] 蔡淑娴 吴香兰[1,2] 金莉莎[1,2] 林勇[1,2] 

机构地区：[1]湖南农业大学茶学教育部重点实验室,湖南长沙410128 [2]国家植物功能成分利用工程技术研究中心,湖南长沙410128

出　　处：《茶叶科学》2013年第4期289-294,共6页Journal of Tea Science

基　　金：国家自然科学基金(31100501;31100502);教育部创新团队计划项目(201042);国家茶叶产业技术体系项目(CARS-23-C);教育部高校博士点专项基金项目(20114320120004)

摘　　要：为了比较提前2 h给予、同时给予及推后2 h给予大红袍水提物对小鼠急性酒精性肝损伤的抵御效果,将50只小鼠随机分成5组:对照组给予等量的蒸馏水,乙醇组一次性给予60%乙醇(0.01 mL/g),提前组、同时组及推后组分别在灌喂酒精前2 h、灌喂酒精中和灌喂酒精后2 h给予大红袍水提物。通过检测小鼠体重、肝重以及血清及肝匀浆中谷草转氨酶(AST)、谷丙转氨酶(ALT)、总胆固醇(TC)、甘油三酯(TG)、丙二醛(MDA)、总巯基等相关指标,并采用HE染色观察肝组织脂肪变性程度。结果表明,一次性豪饮乙醇的急性酒精性肝损伤小鼠模型造模成功,小鼠肝脏组织细胞、血清的生化代谢指标、抗氧化指标及显微观察结果都显示,提前组、同时组和推后组均能不同程度地抵御小鼠急性酒精性肝损伤,以提前组效果最佳,同时组次之,推后组效果不太明显。In order to compare the effect of Dahongpao tea water extract feeding at different time on alcohol-induced acute liver injury in mice. Fifty mice were randomly divided into five groups and were intragastrically given 60% ethanol （0.01 mL/g） alcohol （alcohol group） or distilled water （normal control）. Dahongpao tea water extract was fed at different time, including feeding 2 hours before drinking, feeding while drinking and feeding 2 hours after drinking alcohol. All mice were sacrificed to take serum samples for biochemical measurements, such as glutamic oxalacetic transaminase （AST）, glutamic-pyruvic transminase （ALT）, total cholesterol （TC）, total triglyceride （TG）, malondialdehyde （MDA） and total sulfydryl, liver samples for microexamination using HE staining to determine the degree of fatty changes. A mice model of alcohol induced acute liver injury has been successfully established biochemicalmetabolism index, antioxidant index and microexamination results in mice liver showed that Dahongpao tea water extract fed at different time is able to protect mice from alcohol-induced acute liver injury in different degree. Fed 2 hours before drinking is of best protective effect, and fed while drinking is the second, However, the effect of fed 2 hours after drinking is not significant.

关 键 词：大红袍水提物 给予方式 急性酒精性肝损伤 

分 类 号：R285.5[医药卫生—中药学]