纳米银克拉霉素复合颗粒对体外HeLa细胞毒性的分析  

The cytotoxicity research of nano silver clarithromycin composite particles in vitro

作　　者：王庭阔[1] 姚祖福[2] 刘荣[3] 谭章梅[1] 彭冬梅[1] 廖天义[1] 

机构地区：[1]清远市人民医院耳鼻咽喉-头颈外科,广东清远511500 [2]怀化医学高等专科学校药学系,湖南怀化418000 [3]清远市人民医院妇产科,广东清远511500

出　　处：《湘南学院学报（医学版）》2013年第2期5-7,共3页Journal of Xiangnan University（Medical Sciences）

基　　金：广东省清远市科技局项目(2011B011112082)

摘　　要：目的观察自制纳米银克拉霉素复合颗粒对体外Hela细胞的毒性,为纳米银克拉霉素复合颗粒的临床应用提供基础资料。方法将培养的HeLa细胞分为5组,A组:空白对照;B组:加入PBS;C组:纳米银终浓度10μg/ml;D组:克拉霉素终浓度1μg/ml;E组:纳米银终浓度10μg/ml,克拉霉素终浓度1μg/ml。5组均培养1周,每天进行细胞形态观察、细胞计数及Hochest染色计算凋亡率。结果5组HeLa细胞在第1、2、3、4、5、6及7天,在细胞数量、细胞形态及凋亡率方面比较无显著性差异(P>0.05)。结论自制纳米银克拉霉素复合颗粒对体外Hela细胞无毒性。Objective To observe the cell toxicity of nano - silver elarithmmycin compound granule and pro- vide basic data for the clinical applications. Methods Cultured HeLa cells were divided into five groups, Group A： as control; Group B： PBS; Group C： nano - silver with 10 μg/ml final concentration; Group D： clarithromy- cin with 1μg/ml final concentration; Group E： nano - silver with 10μg/ml final concentration and clarithromycin 1μg/ml. All groups were cultured 7 days, While cell count, cell observation and Hochest staining were done every day. Results There is no obvious differences in cell number, cell morphology and staining positive rate among 5 groups. Conclusion Composite particles of homemade nano silver clarithromycin was no cytotoxicity to Hela ceils.

关 键 词：纳米银 克拉霉素 细胞毒性 HELA细胞 

分 类 号：R318.08[医药卫生—生物医学工程]