舞毒蛾地理种群微卫星遗传多态性分析  

Genetic diversity analysis of several geographic populations of the gypsy moth Lymantria dispar L. by using SSR markers

作　　者：吴妍[1] 张俊华[2] 张浩[1] 陈乃中[2] 钟勇[1] 李正西[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]中国检验检疫科学研究院植物检疫研究所

出　　处：《植物检疫》2013年第4期24-29,共6页Plant Quarantine

基　　金：林业公益性行业科研专项(200904029)

摘　　要：舞毒蛾是国际上重要的检疫性农林害虫。本研究采用7对微卫星引物对来自内蒙古赤峰市克什克腾旗、河北茅荆坝、吉林长春、河北大仓、新疆阿勒泰市阿苇滩等我国5个重要舞毒蛾地理种群进行了遗传多样性及遗传分化分析。结果显示,所检测的我国5个舞毒蛾地理种群多数遗传位点显示出纯合子缺失和杂合子过剩的特点。同时进化分析结果显示,所研究的5个舞毒蛾种群可分为两大支,其中新疆阿勒泰种群单独为一支,其余种群聚类为另一支。微卫星遗传多态性分析结果说明,所研究的舞毒蛾种群的个体之间发生了广泛的基因流动。舞毒蛾种群间存在活跃的基因流动,提示该物种在我国一直在传播扩散。该研究可为舞毒蛾的检验检疫及其防控提供依据。The gypsy moth,Lymantria dispar,is one of the important quarantine pests in China.Seven pairs of primers were used to analyze the genetic diversity and genetic differentiation of the five populations of the gypsy moth from Inner Mongolia,Jilin,Hebei and Xinjiang.The results indicated that there existed a lack of homozygotes and excess of heterozygotes in all of the five geographic populations.Meanwhile,evolutionary analysis showed that the 5 gypsy moth populations diverged into 2 groups,among which the Xinjiang population was placed in a separate group,while the others formed another group.Microsatellite-based genetic diversity analysis suggested that gene flow occurred extensively in the five geographic populations examined.That active gene flow still exists in chinese geographic populations of the gypsy moth implies that the spreading of this species was still happened in China.The data obtained may provide a basis for the quarantine and control of the gypsy moth in our country.

关 键 词：舞毒蛾 微卫星标记 遗传多态性 基因流动 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治] S41[农业科学—植物保护]