甲酸乙酯对松材线虫的熏蒸效果  

Study on the fumigation of ethyl formate against Bursaphelenchus xylophilus

作　　者：詹开瑞[1] 游源浅[1,2] 张晓燕[1] 陈艳[1] 

机构地区：[1]福建出入境检验检疫局,福建福州350001 [2]福建农林大学植物保护学院,福建福州350002

出　　处：《生物安全学报》2013年第2期127-131,共5页Journal of biosafety

基　　金：国家质检总局科技计划项目(2008IK245);福建检验检疫局科技计划项目(FK2006-20)

摘　　要：【背景】在溴甲烷面临禁用的情形下,探讨新熏蒸剂甲酸乙酯对松材线虫的处理效果,可以科学地评估甲酸乙酯的使用前景。【方法】采用松材线虫分离液和带疫松木段,设置甲酸乙酯5个剂量梯度、5个处理温度和5个处理时间,测定其对松材线虫的毒力及CT值。【结果】在25℃下处理3、6、12、24、48h,甲酸乙酯对松材线虫的LC50分别为2.63、1.60、0.99、0.41、0.20mg·L-1。温度对毒力有显著影响,在10～29℃,随温度升高,甲酸乙酯对松材线虫的毒力降低,19和29℃下,1.85mg·L-1甲酸乙酯处理松材线虫的死亡率分别为63%和100%。甲酸乙酯熏蒸12h内,能完全杀灭木段中的松材线虫。在23℃下处理6和12h,松材线虫死亡率达到99%时,甲酸乙酯的CT值分别为453.94和424.14mg·h-1·L-1。【结论与意义】甲酸乙酯可用于松材线虫的检疫处理。[ Background ] Studying the new fumigant, ethyl formate against Bursaphelenchus xylophilus can be useful in the advent of methyl bromide being phased out. [ Method] The toxicity and concentration-time product of ethyl formate were tested indoor against nematode isolated from pine wood. [ Result] The LCso of B. xylophilus to ethyl formate in 3,6, 12, 24 and 48 h at 25℃ were 2.63, 1.60, 0.99, 0.41 and 0.20 mg·L-1, respectively. The toxicity of ethyl formate was significantly affected by temperature, with B. xylophilus mortality decreasing from 63% at 19℃ to 100% at 29℃ for a concentration of 1.85mg·L-1 ethyl formate treatment. The ethyl formate fumigation could completely kill nematodes with 12 hours exposure time. The LCt99 of ethyl formate for B. xylophilus were 453.94 mg·h·L-1 after 6 h exposure and 424.14 mg·h·L-1 after 12 h exposure. [ Conclusion and significance ] The results confirm that ethyl formate could be used as a fumigant against B. xylophilus.

关 键 词：甲酸乙酯 松材线虫 熏蒸 

分 类 号：S763.7[农业科学—森林保护学]