刺槐花中黄酮类成分的高效液相色谱-质谱联用技术分析  

Analysis of Flavonoids of Flower of Robiniapseu Doacacia L. by HPLC-DAD-ESI-MS/MS

作　　者：王岚 王雪芹 仲浩[2] 

机构地区：[1]德州市疾病控制中心,山东德州251200 [2]山东省医学科学院药物研究所,山东济南250062

出　　处：《食品与药品》2013年第4期240-242,共3页Food and Drug

基　　金：山东省科技发展计划(2010GSF10279)

摘　　要：目的高效液相色谱-质谱联用技术分析刺槐花中的黄酮类成分。方法用安捷伦Zorbax SB-柱(250 mm×4.6 mm,5μm),以乙腈-水(1.0%醋酸)为流动相分离花中的黄酮苷,利用质谱的裂解情况进行化合物的结构鉴定。结果鉴定了11种黄酮苷。结论为刺槐花的活性成分鉴定提供了一个简单易行的方法。Objective To analyze the flavonoids of the flower of Robiniapseu doacacia L. by HPLC-DAD-ESI-MS/ MS （DAD：diode array detection）. Methods An Agilent Zorbax SB-Aq cohmm （250 mm × 4.6 ram, 5 μm） was used to separate those flavonoids in the flower. The mobile phase consisting of water containing 1.0 % acetic acid and acetonitrile was used for gradient elution. The flow rate was 1.0 mL/min. ESI-MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition, 11 flavonoid glycosides in the flower of Robiniapseu doacacia L. were identified by mass spectrometry and structure-relevant fragment ions. Conclusion The method of HPLC-DAD-ESI-MS/MS can be simple and reliable to identify the chemical constituents of the flower of Robiniapseu doacacia L.

关 键 词：刺槐花 黄酮类 高效液相色谱-质谱联用 

分 类 号：R284.1[医药卫生—中药学]