核仁素在甲状腺乳头状癌中表达的临床意义  

Clinical significance of nucleolin expression in thyroid papillary carcinoma

作　　者：吕顺增[1] 赵婧[1] 田铧[1] 李菲[1] 李贵宝[1] 房云海[2] 扈燕来[1] 尹群生[1] 

机构地区：[1]山东大学医学院解剖学与组织学胚胎学研究所,济南250012 [2]山东省血液中心血友病诊疗中心,济南250012

出　　处：《解剖学报》2013年第4期498-502,共5页Acta Anatomica Sinica

基　　金：山东省自然科学基金资助项目(ZR2009CZ014;ZR2010HM047)

摘　　要：目的探讨核仁素(NCL)在甲状腺乳头状癌中的表达及临床意义。方法采用免疫组织化学SP染色法检测60例正常甲状腺组织与甲状腺乳头状癌中NCL、趋化因子受体-7(CCR7)与基质金属蛋白酶-9(MMP-9)的表达,分析NCL、CCR7与MMP-9的表达与性别、年龄、肿瘤大小与有无转移的关系以及核仁素与CCR7、MMP-9表达的相关性。结果 NCL在正常甲状腺及甲状腺乳头状癌中的总阳性率分别为0、100%。NCL在伴随转移组癌组织中表达于细胞核、细胞质与细胞膜;NCL在无转移组癌组织中仅表达于细胞核,差异有统计学意义(P<0.05)。不同年龄、性别、肿瘤大小的NCL表达率间的差异无统计学意义(P>0.05)。核仁素细胞核外阳性表达与CCR7与MMP-9的阳性表达呈正相关(P<0.01)。结论 NCL在甲状腺乳头状癌中表达,表达部位在转移与非转移组明显不同,且与CCR7、MMP-9表达有密切关系。核仁素可作为区别癌转移与非转移的标志。Objective To explore the expression of nucleolin in thyroid papillary carcinoma, and discuss its clinical significance. Methods Nucleolin, chemokine receptor 7 （ CCR7 ） , matrix metalloproteinase 9 （ MMP-9 ） were detected by immunohistochemical technique （SP） in 60 cases of normal thyroid tissues and 60 cases of thyroid papillary carcinoma tissues. The relationship among the expressions of nucleolin, CCR7, and MMP-9 and gender, age, or tumor size was analyzed. The correlation between nucleolin and CCRT/MMP-9 was analyzed. Results The expression rate of nucleolin in normal thyroid and thyroid papillary carcinoma tissues was 0, 100% respectively. The expression of nucleolin in thyroid papillary carcinoma with lymph node metastasis was mainly located in nucleus, cytoplasm and cell membrane while the expression of nucleolin was only located in nucleus in thyroid papillary carcinoma tissues without lymph node metastasis. Differences were statistically significant（ P 〈 0. 05 ）. No significant correlation was found between the expression of nucleolin and gender, age or tumor size （P 〉 0. 05）. In thyroid papillary carcinoma, the extra-nuclear expression of nucleolin was positively correlated with the expression of CCR7 and MMP-9（P 〈 0.01 ）. Conclusion Our study suggests that nucleolin can express in thyroid papillary carcinoma and the distribution of nucleolin differs between metastatic carcinoma tissues and non-metastatic carcinoma. Nucleolin is closely associated with CCR7 and MMP-9. NCL can be used as an important indicator for cancer metastasis.

关 键 词：核仁素 趋化因子受体-7 基质金属蛋白酶-9 甲状腺乳头状癌 免疫组织化学 人 

分 类 号：R581.9[医药卫生—内分泌]