整合素CD11a、CD11b和CD11c在大鼠心脏发育中的表达变化  

Expression of integrin CD11a,CD11b and CD11c during the rat heart development

作　　者：穆灵敏[1] 王文锋[2] 郭志坤 张光谋[2] 

机构地区：[1]新乡医学院形态学实验室 [2]新乡医学院生命科学技术系 [3]河南省医用组织再生重点实验室,河南新乡453003

出　　处：《解剖学报》2013年第4期525-529,共5页Acta Anatomica Sinica

基　　金：新乡市科技局立项课题资助项目(09S081)

摘　　要：目的探讨整合素CD11a、CD11b和CD11c在大鼠心脏发育中的表达变化。方法利用免疫组织化学和RT-PCR方法,检测胚胎18d(E18d)、生后5d(P5d)、P19d、P40d及生后1年(P1y)大鼠心肌组织的CD11a、CD11b和CD11c的基因和蛋白表达。结果免疫组织化学结果显示,大鼠心肌组织CD11a、CD11b和CD11c表达部位在心肌细胞质内;从E18d到P1y大鼠心肌组织CD11a、CD11b和CD11c的表达逐渐减弱。RT-PCR显示,CD11a、CD11b、CD11c各组均呈阳性表达。其中CD11a在P5d和P40d间,P5d和P19d间比较(P>0.05)差异无统计学意义,其他各组间比较差异均有统计学意义;CD11b在E18d、P5d、P19d和P40d分别比较(P>0.05)差异无统计学意义,其他各组差异均有统计学意义;CD11c各组间差异有统计学意义(P<0.01)。结论 CD11a、CD11b和CD11c在大鼠心肌的发育过程中出现表达量的变化,不同结构的整合素分子在心脏发育过程表现出相似的表达规律,它们可能对心肌细胞的发育起重要的调控作用。Objective To study the expression changes of integrins CDlla, CDllb and CDllc during the rat heart development. Methods By using RT-PCR and immunohistochemistry, the genes and the protein expression of CD11 a, CD1 l b and CD1 l c of rat cardiac tissue were detected on embryonic day 18 （E18d）, postnatal 5 days （PSd）, postnatal 19 days（P19d） , postnatal 40 days（P40d） and postnatal 1 year（Ply）. Results Immunohistochemical results showed that CDlla, CDllb and CDllc expressed in cell plasma of rat heart tissue, and the level of their expression was gradually decreased from E18d to Ply. RT-PCR results showed that the expressions of CDlla, CDllb and CDllc between each group were positive. Among them, the changes of CD11 a transcripts were not significant compared with that of P40d and P19d （ P 〉 0. 05 ） , and there were significant changes among other groups （ P 〈 0.05 ）. The expressions of CD11 b on E18d, PSd, P19d and P40d were of no significant changes compared with each other respectively （P 〉 0. 05 ） , and there was statistical significant in the other groups. The expression of CDllc between groups was statistically significant （P 〈 0. 01 ）. Conclusion The expression of CD11 a, CD11 b and CD11 c exists a quantity change during the development of the rat myocardium, and different structures of integrin molecules showed similar expression rules during the heart development, so they may be play an important role on regulation in the development of myocardial cells.

关 键 词：心脏 整合素CD 免疫组织化学 反转录一聚合酶链反应 大鼠 

分 类 号：R322.1[医药卫生—人体解剖和组织胚胎学]