西花蓟马对多杀菌素的抗性生化机制研究  

Biochemical mechanism of resistance to spinosad in Frankliniella occidentalis

作　　者：侯文杰[1] 李飞[1] 吴青君[1] 徐宝云[1] 张友军[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《应用昆虫学报》2013年第4期1042-1048,共7页Chinese Journal of Applied Entomology

基　　金：973项目(2009CB119004);北京市自然基金(6092018);农业行业科技专项(200803025)

摘　　要：在室内持续用多杀菌素对西花蓟马Frankliniella occidentalis(Pergande)进行抗性汰选,获得抗性倍数达到11 999倍的极高抗品系。该抗性品系对乙基多杀菌素和噻虫嗪具有显著的交互抗性,抗性倍数分别为53 718和84倍,对阿维菌素和毒死蜱的敏感性也有显著下降,交互抗性倍数分别为3.33和2.28倍,对虫螨腈无明显交互抗性。生化测定结果表明,抗性品系的羧酸酯酶、谷胱甘肽S-转移酶和多功能氧化酶的活力与敏感品系均无显著差异,推测抗性可能与靶标位点的敏感性降低有关。Successive generations of the western flower thrips Frankliniella occidentalis （ Pergande ） , were selected for resistance to spinosad. After 5 years we obtained a highly resistant strain, displaying 11 999-fold resistance to spinosad. The resistant strain also showed 53 718- and 84-fold cross-resistance, respectively, to spinetoram and thiamethoxam, low cross-resistance （3.33 and 2.28-fold, respectively） to abamectin and chlorpyrifos, but no observed cross-resistance to chlorfenapyr. Biochemical analysis showed that the activities of carboxylesterase, glutathione S-transferase and multi- functional oxidases in the resistant strain were not significantly different from those of the susceptible strain. These results indicate that metabolic detoxification does not play a role in the resistance of western flower thrips to spinosad, suggesting that spinosad may reduce sensitivities of the target sites.

关 键 词：西花蓟马 多杀菌素 抗性汰选 交互抗性 解毒酶系 

分 类 号：S433[农业科学—农业昆虫与害虫防治]