Lep和LEPR基因在不同脂尾型绵羊脂肪组织中的mRNA表达研究  

Differential Expression of Lepand LEPRmRNA in Adipose Tissues of Sheep with Divergent Fat-tails

作　　者：刘宝凤[1] 周沙沙[1] 王景霖[1] 魏琳琳[1] 乔利英[1] 刘建华[1] 梁琛[1] 刘文忠[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801

出　　处：《畜牧兽医学报》2013年第7期1014-1022,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30972084);山西省科技攻关项目(011029)

摘　　要：本研究利用Real-time PCR技术研究Lep和LEPR2个基因在2个具有显著尾型差异的绵羊品种(广灵大尾羊和小尾寒羊)不同脂肪组织中的发育性表达规律,以探讨这2个基因与绵羊脂肪代谢的关系。采集2个品种2、4、6、8、10和12月龄时共计96个个体(每品种公、母各半)7个部位(大网膜、小网膜、肠系膜、腹膜后、皮下、肾周和尾部)的脂肪组织进行mRNA表达研究。结果表明,Lep和LEPR在广灵大尾羊和小尾寒羊脂肪组织中的表达存在时空差异性。作为主效应,品种和性别基本不影响Lep和LEPR mRNA的表达,但品种与月龄间的互作对这2个基因的表达均有所影响。尽管这2个基因协同调节脂肪沉积和能量代谢,但是存在反向调节。有关结果为深入研究绵羊脂肪代谢的遗传机制提供了重要的科学依据。Real-time PCR technique was used to study the ontogenetic mRNA expression of Lep and LEPR genes in two sheep breeds with distinct tail types （Guangling Large Tailed and Small Tailed Han Sheep）, so that to discuss the probable relationship between the Lep and LEPR mRNA expression and fat metabolism. Adipose tissues from seven parts of the body（great and small omenta, tail, subcutaneous, mesenterium, perirenal and retroperitoneum） were collected from 96 animals （48 for each breed with equal sex ratios） from 2- to 12- month old at the interval of two months to study the mRNA expression. The results showed that both Lep and LEPR expressed spatiotemporally in the two breeds. As the main effects, sex and breed did not basically influenced the expression of Lep and LEPR, but the breed × age interaction affected the expression of the two genes to different extents. Although the two genes had a synergistic effect on the regulation of fat deposition and energy metabolism, their expressions were inversely regulated. These results provide the important scientific bases for further research on the genetic mechanism of fat metabolism in sheep.

关 键 词：绵羊 LEP LEPR MRNA表达 脂肪组织 

分 类 号：S826[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]