H9N2亚型AIV全基因序列分析及重组病毒R01/NASS气源性传播的特点  

Whole Genome Sequence Analysis of H9N2 AIV and the Characteristic of Airborne Transmission of the Recombinant Virus R01/NASS

作　　者：魏宝之[1] 杨燕[1] 胡家卿[1] 吕静[1] 柴同杰[1] 

机构地区：[1]山东农业大学动物科技院,泰安271018

出　　处：《畜牧兽医学报》2013年第7期1099-1108,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑项目(2012BAD39B02);山东农业大学2012博士后基金项目

摘　　要：为了研究H9N2亚型禽流感病毒(avian influenza virus,AIV)气源性传播的分子机制,本研究以1株2008年山东省分离的H9N2亚型AIV为研究对象,对其全基因组序列进行分子特性和遗传进化分析;利用反向遗传操作技术,对具有气源性传染能力的山东分离株(SD01株)和不具有气源性传染能力的广东分离株(SS94株)进行重组,转染细胞拯救出重组株R01/NASS,对其在鸡群间的气源性传染能力进行测定。对SD01株序列分析表明,SD01株的M、NS、NP和HA基因与HK/G9/97相似性最高,NA、PB1和PB2基因与HK/Y280/97相似性最高,PA基因与SH/F/98相似性最高。病毒在SPF鸡群间传播性试验发现,重组病毒R01/NASS接种鸡群后,可以经直接接触途径传染鸡群,但是却未感染气溶胶被动感染组鸡群,表明NA基因的替换使病毒失去了气源性传染的能力。该试验结果为从分子水平上深入研究SD01株AIV NA蛋白氨基酸位点与气源性传染的关系提供了理论依据。In order to investigate the molecular mechanism affecting the airborne transmission for HgN2 avian influenza virus （AIV）, one isolates from Shandong province （SD01） was chose as research subject. The character of its complete nucleotide sequences and phylogenetic tree were analyzed. One recombinant AIV （R01/NASS）, NA gene from Guangdong isolate （SS94） without airborne transmissibility and other seven genes from SD01 with this ability, was generated by reverse genetics and its transmissibility was determined in chickens. The results showed that M, NS, NP and HA genes had the highest homology with HK/G9/97, NA, PB1 and PB2 genes had highest homology with HK/Y280/97, and PA gene with SH/F/98. Transmission experiment indicated that the recombinant AIV could transmit by direct contact, but not by aerosol. These results demonstrated that the replacement of NA gene made SD01 virus lost its airborne transmissibility. The results would be useful for further studies on the key amino acids of NA protein, which may relate to the airborne transmission of H9N2 AIVs.

关 键 词：H9N2亚型AIV 序列分析 反向遗传操作技术 气源性传染 

分 类 号：S852.659.5[农业科学—基础兽医学]