重复性“饥饿/再投喂”对大鼠脂代谢及解偶联蛋白表达的影响  

Effects of repeated fasting/refeeding on lipid metabolism and uncoupling proteins expression in rats

作　　者：吴博[1] 冯彦红 刘重斌[3] 赵惠玲[1] 王一龙[1] 陈锡文[1] 

机构地区：[1]温州医学院实验动物中心,浙江温州325035 [2]石家庄市妇幼保健院,河北石家庄050091 [3]湖州师范学院医学院生理教研室,浙江湖州313000

出　　处：《中国应用生理学杂志》2013年第4期363-367,I0003,共6页Chinese Journal of Applied Physiology

基　　金：浙江省实验动物科技计划项目(2009F80024)

摘　　要：目的:探讨经过重复性"饥饿/再投喂"饲喂方式处理的大鼠再摄取高脂食物时,脂肪组织解偶联蛋白2(UCP2)和肌肉组织解偶联蛋白3(UCP3)基因及蛋白的表达变化及其对脂代谢可能的作用机制。方法:采用"重复性饥饿1天/再投喂1天,持续6周"的方式(基础饲料)饲喂模型动物,6周末实验组(RFR)大鼠改为每天饲喂高脂饲料(RFR-CF/HF),并将对照组随机分为两组,一组改喂高脂饲料(HF),另一组继续饲喂基础饲料(CF)至第12周。分别于实验第6周和第12周末处死动物,腹主动脉采血后分离血清和血浆,检测血脂、血清葡萄糖、游离脂肪酸及血浆胰岛素的含量,HE染色观察肝组织形态学的变化,RT-PCR和Western blot法检测脂肪组织UCP2和肌肉组织UCP3基因及蛋白的表达水平。结果:①RFR组血清葡萄糖含量显著低于对照组(P<0.05),游离脂肪酸(FFA)含量、UCP2mRNA、UCP3mRNA和蛋白表达水平则均显著高于对照组(P<0.05)。②RFR-CF/HF组血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和血浆胰岛素含量均显著低于HF组而高于CF组(P<0.05),血清FFA含量显著低于HF组和CF组(P<0.01),UCP2和UCP3mRNA及蛋白表达水平均显著高于HF组而低于CF组(P<0.05)。结论:重复性"饥饿/再投喂"可减轻高脂饮食诱导的肥胖程度,增加大鼠脂肪组织UCP2和肌肉组织UCP3基因及蛋白的表达,上调由肥胖引起的质子泄漏,提高大鼠的基础能量代谢率。Objective： To explore the expression changes of mRNA and protein of uncoupling protein 2（UCP2） in adipose tissues and uncoupling protein 3（UCP3） in muscle tissues of rats which were treated with repeated fasting/refeeding and followed by fed with high-fat diet,and their possible mechanism on lipid metabolism.Methods： The model of repeating fasting/refeeding rats（repeated cycles of 1-day fasting and 1-day refeeding for 6 weeks fed with common-fat diet,RFR） was designed.At the end of the 6th week,the RFR rats were switched to high-fat diet every day（RFR-CF/HF）.Moreover,the control rats were randomly divided into two groups and then fed with high-fat diet（HF） and common-fat diet（CF） respectively for 6 weeks.All rats were killed at the end of the 6th and the 12th week,serum and plasma samples were taken from abdominal aorta,and then the concentration of serum lipids,glucose,free fatty acid（FFA）,and plasma insulin were measured.The histomorphological changes of liver tissues were observed by HE staining.The expression level of mRNA and protein of UCP2 in adipose tissues and UCP3 in muscle tissues was respectively measured by RT-PCR and Western blot.Results： ①The concentration of serum glucose in RFR group was significantly lower than that in control group（P＆lt;0.05）,while the concentration of serum FFA,expression level of UCP2 mRNA,UCP3 mRNA and protein were significantly higher than those in control group（P＆lt;0.05）.②The concentration of serum total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein cholesterol（LDL-C）,and plasma insulin in RFR-CF/HF group was significantly lower than that in HF group,but significantly higher than that in CF group（P＆lt;0.05）.The concentration of serum FFA was significantly lower than that of HF and CF groups（P＆lt;0.01）.The expression level in UCP2,UCP3 mRNA and protein was significantly higher than that of HF group,but significantly lower than that of CF group（P＆lt;0.05）.Conclusion： The feeding pat

关 键 词：饥饿 再投喂 肥胖 解耦联蛋白 游离脂肪酸 

分 类 号：R589.2[医药卫生—内分泌]