淫羊藿苷对自然衰老大鼠海马组织BDNF和TrkB mRNA表达的影响  

Effects of icariin on expression of BDNF and TrkB mRNA in natural aging rat hippocampus

作　　者：吴芹[1] 陆远富[1] 张锋[1] 龚其海[1] 李菲[1] 钱英[2] 谭祈军[2] 石京山[1] 

机构地区：[1]遵义医学院贵州省基础药理重点实验室暨药理学教研室,贵州遵义563000 [2]遵义医学院药学院,贵州遵义563000

出　　处：《中国新药与临床杂志》2013年第7期548-551,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：国家自然科学基金(81160400);973计划前期研究专项(2011CB512009);教育部长江学者和创新团队发展计划(IRT1197)

摘　　要：目的观察淫羊藿苷对自然衰老大鼠海马组织BDNF和TrkB mRNA表达的影响。方法自然衰老大鼠(24月龄)15只,随机分为对照组、淫羊藿苷低剂量和高剂量组,分别灌胃给予蒸馏水(1 mL.kg-1)和淫羊藿苷30 mg.kg-1、60 mg.kg-1,连续给药60 d。观察海马组织形态学变化;实时荧光定量RT-PCR法检测各组大鼠海马组织中BDNF、TrkB mRNA的表达。结果 HE染色显示对照组老年大鼠海马神经元稀疏,排列紊乱,并散在变性的神经元。给予淫羊藿苷后海马神经元排列较整齐,变性神经元减少,海马组织BDNF和TrkB mRNA表达量(306±59和389±80)与对照组(61±5和97±25)比较明显升高(P<0.05)。结论淫羊藿苷抗衰老作用一定程度上是通过上调海马组织中BDNF和TrkB mRNA表达实现的。AIM To investigate the effects of icariin （ICA） on the expression of BDNF and TrkB mRNA in the hippocampus of natural aging rats. METHODS Fifteen 24- month- old rats were randomly divided into control group and ICA low dose （30 mg·kg^-1） and high dose （60mg·kg^-1） groups, ig treated with distilled water and ICA respectively for 60 days. The morphological changes of hippoeampal neurons were observed by HE staining and the mRNA levels of BDNF and TrkB were detected by real-time fluorescent quantitative RT-PCR. RESULTS HE staining indicated that the number of neurons reduced and the neuronal arrangement was disordered with neuronal degeneration in the natural aging rats. ICA treatment could improve the arrangement of neurons and attenuate the neuronal degeneration. Compared with the control group, ICA apparently increased the expression of BDNF and TrkB mRNA in the hippoeampus of aging rats. CONCLUSION The anti-aging effects of ICA might be related to the increased BDNF and TrkB mRNA expressions in the hippoeampus.

关 键 词：淫羊藿苷 衰老 大鼠 海马 脑源性神经营养因子 受体 TrkB 

分 类 号：R969.1[医药卫生—药理学]