2011-2012年海南地区淋球菌β-内酰胺酶的检测及其基因分型  

β-Lactamases and Genotyping of Neisseria gonorrhoeae in Hainan Area from 2011-2012

作　　者：钟娜[1] 乔凤[1] 刘巧[1] 许玉军[1] 裴东怒[1] 郑文爱[1] 王芳乾[1] 

机构地区：[1]海南省皮肤性病防治中心,海南海口507206

出　　处：《中国皮肤性病学杂志》2013年第8期778-779,782,共3页The Chinese Journal of Dermatovenereology

基　　金：海南省自然科学基金资助项目(811171)

摘　　要：目的了解质粒介导的产青霉素酶淋球菌(PPNG)在海南地区的分子流行病学情况。方法采用纸片酸度法测定产β-内酰胺酶菌株(PPNG),PCR方法鉴定β-内酰胺酶质粒并进行TEM-1基因质粒分型。结果 2011-2012年共检测了214株淋球菌,检出PPNG65株(31.31%),其中55株(84.62%)携带TEM-1基因亚洲型质粒,10株(15.38%)携带TEM-1基因非洲型质粒;未见多伦多型和里约型。结论海南地区流行的PPNG以亚洲型TEM-1基因为主(84.62%),非洲型其次(15.38%)。Objective To investigate the molecular epidemiology of plasmid-mediated penicillinase producing Neisseria gonorrhoeae （PPNG） isolated from Hainan area. Methods Penicillnase（β-lactamase） producing Neisseria gonorrhoeae was analyzed by paper acidometric method, and a PCR method was used to distinguish subtype of TEM-1. Results 214 clinical strains of Neisseria gonorrhoeae were isolated and tested from Hainan area in 2011 -2012. 65 strains （31.31%） was detected to be PPNG. Subtype in TEM-1 gene of β-1actamase plasmids of PPNG showed that 55 （84. 62% ） PPNGs carried Asian-type plasmids, and 10 （15.38%） PPNGs carried African-type plasmids. Toronto-type and Rio-type plasmids were not found. Conclusion A- mong PPNG which were prevalent in Hainan area, the Asian-type （ 84.62% ） β-1actamase plasmids were dominated, while African-type（ 15.38% ） plasmids were sporadic.

关 键 词：奈瑟淋球菌 产青霉素酶淋球菌(PPNG) TEM-1基因 

分 类 号：R759.2[医药卫生—皮肤病学与性病学]