姜黄素对胆管癌MZ-Cha-1细胞凋亡的诱导作用  

Effect of curcumin on proliferation of human bile duct carcinoma MZ-Cha-1 cells

作　　者：杨海波[1] 宋巍[1] 陈兰英[1] 

机构地区：[1]河南城建学院生命科学与工程学院,河南平顶山467036

出　　处：《癌变．畸变．突变》2013年第4期289-292,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：河南省重点科技攻关项目(092102310006);河南省教育厅基金项目(2009B180001)

摘　　要：目的:研究姜黄素对胆管癌MZ-Cha-1细胞凋亡的诱导作用。方法:用0、10、20、40μg/mL姜黄素处理MZ-Cha-1细胞12、24、36h后,应用噻唑蓝(MTT)检测姜黄素对MZ-Cha-1细胞的抑制情况,再选择10μg/mL姜黄素处理MZ-Cha-1细胞24h后应用Giemsa和Hoechst33258染色检测MZ-Cha-1细胞的凋亡情况,应用流式细胞术检测细胞周期分布。结果:与对照组相比较,各姜黄素处理组对胆管癌MZ-Cha-1细胞有明显的抑制作用,并显示出对剂量和时间的依赖效应(P<0.05)。10μg/mL姜黄素诱导处理24h后,经Giemsa染色显示细胞体积缩小、核固缩、染色质凝聚等显著的凋亡特征;细胞核经Hoechst33258染色出现浓染致密的固缩形态和颗粒状荧光;细胞周期检测出现细胞被阻滞在G0/G1期,G2/M期细胞较对照组显著减少(P<0.05)。结论:姜黄素能够有效诱导人胆管癌MZ-Cha-1细胞的凋亡。OBJECTIVE： To study the apoptotic effects of human bile duct carcinoma MZ-Cha-1 cells induced by curcumin. METHODS： After the MZ-Cha-1 cells induced by 0, 10, 20 and 40 μg/mL curcumin for 12, 24, 36 h, the apoptotic effects of curcumin were studied using the methods of MTT to detect the curcumin on MZ- Cha-1 cell suppression, and selected 10μg/mL of curcumin treatment MZ-Cha-1 cells after 24 h, using Giemsa staining and Hoechst33258 dye to assess MZ-Cha-1 cell apoptosis, using flow cytometry to detect the cell cycle distribution. RESULTS： Compared with control group, the eureumin on MZ-Cha-1 bile duct carcinoma cell had obvious inhibitory effect, and in dose- and time- dependent mamer（P〈0.05）. Proliferation of MZ-Cha-1 cells could be inhibited by 10μg/mL curcumin after 24 h. Light microscope showed that the morphology of the cells treated with curcumin appeared shrunken. Concentrated cell nucleus appeared granular and fluorescent by Hoechst33258 staining. Flow cytometry analysis showed that G0/G1 phase was increased and G2/M phase decreased （P〈0.05）. CONCLUSION： This study suggested that cureumin could induce apoptosis of the human MZ-Cha-1 cells effectively.

关 键 词：姜黄素 胆管癌细胞 细胞凋亡 

分 类 号：R965[医药卫生—药理学]