乳酸钠对大鼠的亚慢性经皮毒性试验  

Subchronic dermal toxicity study of sodium lactate in rats

作　　者：林飞[1] 单纯[1] 许建霞[1] 吕建军[1] 

机构地区：[1]中国食品药品检定研究院,北京100050

出　　处：《癌变．畸变．突变》2013年第4期297-301,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:观察大鼠连续经皮给予大剂量乳酸钠后的毒性靶器官及其损害的可逆性。方法:设溶剂对照组和乳酸钠高、中、低3个剂量组(分别为2000、1000和500mg/kg),实验组大鼠连续经皮涂抹乳酸钠90d,溶剂对照组给予蒸馏水。于第7周、第13周和停止染毒后4周,每组剖杀部分大鼠做血液学、生化学、眼科、尿液检测,测量脏器湿重和脏器系数,并进行解剖病理学观察。结果:染毒第13周各剂量组大鼠血液钠离子、钙离子浓度升高,钾离子、氯离子浓度降低,膀胱腔内存在蛋白样物质聚集;高剂量组大鼠尿液中蛋白和胆红素浓度升高,比重降低。停止染毒后4周高剂量组大鼠血液钠离子浓度仍然较高。结论:乳酸钠染毒后大鼠出现电解质轻度紊乱和尿液异常、尿蛋白增加等变化,因此乳酸钠对大鼠具有一定的毒性作用。OBJECTIVE： To study the subchronic dermal toxicity of sodium lactate in rats, and analyze the target organ damage and its reversibility. METHODS： Sodium lactate was given to rats by transdermal administration at dose of 2 000, 1 000 and 500 mg/kg for 90 days. Some rats from each group were sacrificed at 7^th, 13th week and 4 weeks after recovery, blood count and biochemistry were tested, ophthalmic and urinalysis tests were performed, wet weight of individual organs and the coefficient values were determined, and anatomic pathology were observed. RESULTS： With all treatment doses, K＋, Cl- decreased and Na＋, Ca^2＋ increased, protein precipitation was found in the bladder lumen. High dose group showed urinary protein and bilirubin concentration increased, specific gravity reduced. The above had dose-response relationship. Na＋ values in high dose group remained elevated in the recovery stage. CONCLUSION： Sodium lactate showed significant toxic effects in rats as determined by mild disturbance of electrolyte and abnormal urinary protein. [

关 键 词：亚慢性经皮毒性 乳酸钠 大鼠 电解质 尿蛋白 

分 类 号：R994.3[医药卫生—毒理学]