左、右归丸及其拆方含药血清对骨髓间充质干细胞增殖和成骨诱导的影响  

Effect of Zuogui pill,Yougui pill,and their containing serum on the proliferation and osteogenic induction of bone marrow mesenchymal stem cells

作　　者：何文智[1] 宋囡[2,3] 王智民[4] 任艳玲[2] 

机构地区：[1]辽宁中医药大学药学院,沈阳110032 [2]辽宁中医药大学基础医学院 [3]辽宁中医药大学附属第二医院 [4]辽宁中医药大学第一临床学院

出　　处：《中国骨质疏松杂志》2013年第7期697-702,680,共7页Chinese Journal of Osteoporosis

基　　金：辽宁省教育厅创新团队项目(LT2010068);辽宁省高等学校优秀人才支持计划(LR201025);辽宁省科技厅自然科学基金项目计划(201102148)

摘　　要：目的观察左、右归丸及其拆方含药血清对体外培养的大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)增殖和向成骨细胞(osteoblast,OB)诱导的影响。方法运用全骨髓贴壁法分离和培养大鼠BMSCs;分别以左归丸、右归丸、两方共同药、滋肾阴药、补肾阳药、阳性对照药补佳乐制备的大鼠含药血清加诱导剂(地塞米松、维生素C、β-甘油磷酸钠)、诱导剂和空白含药血清组共8组对BMSCs进行干预,采用MTT法检测BMSCs增殖和左、右归丸及其拆方含药血清对BMSCs增殖的影响;流式细胞仪对BMSCs进行鉴定;用PNPP法检测左、右归丸及其拆方含药血清对BMSCs向OB诱导的影响。结果 P4代以后细胞细胞表型CD90呈阳性表达,CD11b/c、CD45呈阴性表达。左、右归丸及其拆方含药血清对BMSCs增殖和成骨诱导都具有促进作用,左归丸、右归丸、滋肾阴药组与其他组比较效果显著(P<0.05)。结论左、右归丸及其拆方含药血清可以协同诱导剂对BMSCs增殖和成骨诱导具有显著的促进作用。Objective To observe the effect of Zuogui pill, Yougui pill, and their containing serum on the proliferation of rat bone marrow mesenehymal stem cells （BMSCs） cultured in vitro and the induction of BMSCs differentiated into osteoblasts （OB）. Methods Rat BMSCs were isolated using adherence method and then cultured with different medium. Serum containing Zuogui pill, Yougui pill, both of Zuogui and Yougui pill, nourishing the kidney drugs, tonifying the kidney drugs, and positive control drugs （Bujiale） was collected. Then, BMSCs were cultured with the drug containing- serum plus inductive agents （including dexamethasone, vitamin C, and [3-sodium glycerophosphate） , inductive agents, or drug-containing serum. The proliferation of BMSCs was detected using MTT method. BMSCs were identified using flow cytometry. The effect of Zuogui pill, Yougui pill, and their containing serum on inducing BMSCs differentiating into OB was detected using PNPP method. Results At the 4th passage, CD90 was positive, while CDllb/c and CD45 were negative at the surface of the cells. Zuogui pill, Yougui pill, and their containing serum could significantly promote the proliferation and osteogenic induction of BMSCs. Zuogui pill, Yougui pill, and nourishing the kidney drugs had significant effect when compared with others （P 〈 0. 05）. Conclusion Zuogui pill, Yougui pill, and their containing serum, plus with inductive agents, all could significantly promote the proliferation and osteogenic induction of BMSCs.

关 键 词：左归丸 右归丸 骨髓间充质干细胞 细胞增殖 成骨诱导 

分 类 号：R681[医药卫生—骨科学]