53个小麦品种中1BL/1RS易位系的分子检测  

Detection of 1BL/1RS by Molecular Markers in 53 Wheat Varieties

作　　者：魏学军[1] 张娜[1] 胡亚亚[1] 王飞[1] 彭巧慧[2] 杨文香[1] 

机构地区：[1]河北农业大学植物保护学院,河北省农作物病虫害生物防治工程技术研究中心,保定071000 [2]河北省农林科学院,石家庄050051

出　　处：《分子植物育种》2013年第4期503-508,共6页Molecular Plant Breeding

基　　金：农业部公益性行业(农业)科研专项经费项目(200903035-14);国家重点基础研究发展计划(2013CB127700)共同资助

摘　　要：为合理利用小麦种质资源,快速准确检测小麦品种中的1BL/1RS易位系,采用ω-secalin和Glu-B3两个特异性分子标记检测了1BL/1RS在53个小麦品种中的分布情况。正相关引物ω-secalin在TcLr26和大粒王等8个品种中检测到1076bp的特异性条带,在Thatcher及其它品种中未检测到特异性条带。负相关引物Glu-B3在TcLr26和大粒王等8个品种中未扩增到636bp条带,Thatcher和其它品种中能扩增出636bp条带。通过两个正负相关标记相互验证,获得一致的结果,表明大粒王、鲁夫16系、太空8号、太育28、温麦8号、冀麦30、北京837、牛朱特是1BL/1RS易位系。For reasonable utilization of wheat germplasm resources, rapid and accurate detection of wheat 1BL/1RS translocation lines, 53 wheat varieties were detected by ω-secalin and Glu-B3 specific molecular markers. 1 076 bp specific band can be amplificated in TcLr26, Daliwang and other seven varieties by positive primer ω-secalin. 636 bp band can be detected by negative primer Glu-B3 in the varieties excepted TcLr26, Daliwang and other seven varieties. The results were verified and consistent by using two specific markers ω-secalin and Glu-B3. The results showed that Daliwang, Lufu16 line, Taikong8, Taiyu28, Wenmai8, Jimai30, Beijing837 and Neuzucht were 1BL/1RS chromosome translocation lines.

关 键 词：小麦 1BL 1RS 抗性 STS分子标记 

分 类 号：S512.1[农业科学—作物学]