不同单株叶籽银杏DNA甲基化MSAP分析  

Analysis on DNA Methylation of Ginkgo biloba L. var. epiphylla Mak. by MSAP

作　　者：王聪聪[1] 李际红[1] 邢世岩[1] 吴岐奎[1] 姚培娟[1] 

机构地区：[1]山东农业大学林学院农业生态与环境实验室,泰安271018

出　　处：《分子植物育种》2013年第4期551-558,共8页Molecular Plant Breeding

基　　金：国家自然基金项目(32070589)资助

摘　　要：利用MSAP(甲基化敏感扩增多态性)技术对11株叶籽银杏的胞嘧啶甲基化水平和模式进行评估。结果表明,叶籽银杏基因组的CCGG序列中检测到36.86%～46.43%的DNA发生甲基化。本研究从36对选扩增引物中筛选出14对引物组合,共得到2879条清晰可辨的带,叶籽银杏总体平均DNA基甲基化水平为42.14%,甲基化模式以内侧胞嘧啶为主,其中,外侧胞嘧啶甲基化水平为11.90%～23.90%,内侧胞嘧啶甲基化水平为17.03%～28.37%。UPGMA聚类分析和主成分分析结果一致,不同单株的叶籽银杏可分为3大类。推断叶籽银杏的变异机制可能与DNA甲基化有关。The level and pattern of cytosine methylation in 11 Ginkgo biloba L. var. epiphylla Mak.. Individual’s genomes were assessed by using the technique of methylation-sensitive amplified polymorphism （MSAP）. The results showed that 36.86%～46.43% DNA methylation levels were detected in the CCGG sequence of Ginkgo biloba L. var. epiphylla Mak. genomes. Total 2 879 distinguishable bands were amplified by using 36 pairs of selective primers, and 14 of the 36 primers showed polymorphism. The average proportion of DNA methylation was 42.14%. The inner-methylation of double-stranded DNA was the main pattern of the genomic DNA methylation on Ginkgo biloba L. var. epiphylla Mak.. Among these, the outer-methylation and inner-methylation level were 11.90%～23.90% and 17.03%～28.37%, respectively. Cluster analysis using UPGMA was consistent with principal components analysis, the results demonstrated the different individuals could be clustered into three groups.

关 键 词：叶籽银杏 DNA甲基化 MSAP分析 表观遗传学 

分 类 号：S792.95[农业科学—林木遗传育种]