番茄花粉管通道遗传转化方法  

Transgenic Technology of Pollen-tube Pathway in Tomato (Solanum lycopersicum M.)

作　　者：金晓霞[1] 李婉婷[1] 于丽杰[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,哈尔滨150025

出　　处：《分子植物育种》2013年第4期605-610,共6页Molecular Plant Breeding

基　　金：黑龙江省教育厅科学技术研究项目(11511122)资助

摘　　要：本研究针对授粉后的转化时机对遗传转化效果的影响,采用两种遗传转化方法(处理Ⅰ:授粉后24h切除花柱进行转化;处理Ⅱ:授粉后12h切除花柱进行转化),利用免疫组织化学技术对番茄花粉管通道遗传转化的途径进行研究。结果表明:处理Ⅰ在转化后26～36h,外源DNA从珠被的一侧进入胚囊;处理Ⅱ在转化后12～38h,外源DNA到达胚珠的珠柄处。外源DNA在番茄花柱的移动速度为1.507±0.105mm/h,其通过花柱的理论时间为4.5h。由此看出,不同的转化操作时机对转化率有影响,认为花粉管通道法转化番茄的适宜时期为授粉后24h进行切除整个番茄花柱的转化操作。Aiming at the influence of transform opportunity after pollination into the effects of genetic transformation, we observed the genetic transformation of tomato pollen tube pathway using immunohistochemical technique which used two kinds of genetic transformation methods （Ⅰ： resection of style in 24 h after pollination for transformation; Ⅱ： resection of style in 12 h after pollination for transformation）. The results showed that, exogenous DNA from one side of the integument into the embryo sac in the transformed 26～36 h in treatment Ⅰ, while in the transformed 12～38 h in treatment Ⅱ, exogenous DNA reached the ovule funiculus. The movement speed of exogenous DNA in tomato style was 1.507±0.105 mm/h, the theory of time through style was 4.5 h. As a result, different transforming time had effect on the transformation rate, and the proper period of pollen-tube pathway transformation of tomato was resection of whole tomato style in 24 h after pollination.

关 键 词：番茄 花粉管通道法 免疫组织化学 

分 类 号：S641.2[农业科学—蔬菜学]