胎盘组织中色素上皮衍生因子蛋白的表达变化与子痫前期发病的关系  

Relationship between pigment epithelium-derived factor expressed in placentas and the pathogenesis of preeclampsia disease

作　　者：吴英[1] 余艳红[1] 钟梅[1] 龚时鹏[1] 李青[1] 刘士三[1] 

机构地区：[1]南方医科大学南方医院妇产科,广州510515

出　　处：《中华妇产科杂志》2013年第7期490-493,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的 探讨胎盘组织中色素上皮衍生因子（PEDF）蛋白表达及其与胎盘血管生成的机制在子痫前期发病中的作用.方法 选取2011年10月至2013年1月在南方医科大学南方医院妇产科住院分娩的子痫前期孕妇60例,分为子痫前期轻度组30例,子痫前期重度组30例；同期分娩的健康妊娠晚期孕妇40例作为对照组.采用蛋白印迹和免疫组化SP法测定各组孕妇胎盘组织中PEDF蛋白的表达,计数胎盘微血管密度（MVD）,对胎盘组织中PEDF蛋白表达与MVD进行相关性分析.结果 （1）胎盘病理：子痫前期轻度组和重度组胎盘重量明显减轻,绒毛血管数量减少,管腔狭窄,滋养细胞基底膜增厚；合体滋养细胞结节状增生,伴灶性梗死、纤维素样坏死或血栓形成.而对照组胎盘组织则无上述病理改变.（2） PEDF蛋白表达：子痫前期轻度组、子痫前期重度组和对照组胎盘PEDF蛋白表达水平分别为0.63 ±0.09、0.93±0.07和0.47 ±0.04,子痫前期轻度组及子痫前期重度组胎盘PEDF蛋白表达水平显著高于对照组,分别比较,差异有统计学意义（P＜0.05）；且子痫前期重度组显著高于子痫前期轻度组,两组比较,差异有统计学意义（P＜0.05）.（3）MVD检测：子痫前期轻度组、子痫前期重度组和对照组胎盘组织中MVD水平分别为106 ±9、93 ±8、136 ±9,子痫前期轻度组及重度组显著低于对照组,分别比较,差异有统计学意义（P＜0.05）,且子痫前期重度组显著低于子痫前期轻度组,两组比较,差异有统计学意义（P＜0.05）.（4）相关性分析：子痫前期轻度组及子痫前期重度组胎盘组织中PEDF蛋白表达与MVD均呈负相关（分别为r=-0.426,P ＜0.05;r=-0.646,P＜0.05）,对照组胎盘组织中PEDF蛋白表达与MVD也呈负相关（r=-0.589,P＜0.05）.结论 子痫前期孕妇胎盘组织中PEDF蛋白高表达,导致胎盘血管生成障碍和胎盘发生缺血缺氧性病理改变,从而参与子痫Objective To investigate the effect of pigment epithelium-derived factor （PEDF） on the pathogenesis of preeclampsia disease,by detecting the expression of PEDF in the placentas,as well as the relationship between PEDF and the production of placental vessels.Methods A study was performed in 60 cases of pregnant women with preeclampsia in the obstetrical department of Nanfang Hospital affiliated to southern medical university from October 2011 to January 2013,in which 30 cases were patients with mild preeclampsia（mPE） and other 30 cases were those with severe preeclampsia （sPE）.40 normal pregnant women who also been hospitalized and delivered were selected as control group.The expression of PEDF and micro-vessel density （MVD） in placentas were assayed by using western blot and SP immunohistochemical method,then the relationship between PEDF and MVD was analyzed.Results （1） The pathological changes of placentas：the placental weight were lightened obviously in the mild preeclampsia and severe preeclampsia groups,the reduced blood vessels and luminal stegnosis were found in chorionic villus,basement membrane of trophocytes were thickening.The hyperplasia syneytiotrophoblast were like nodosity,with focus infarction,fibrinoid necrosis,or thrombogenesis.While there was no the above mentioned pathological alteration in normal control group.（2）The levels of PEDF expression in mild and severe preeclampsia group were 0.63 ± 0.09,0.93 ± 0.07,while 0.47 ± 0.04 in control group,which in mild and sever preeclampsia groups were significantly higher than that in normal group （P 〈 0.05）.Compared to mild preeclampsia group,the expression of PEDF was significantly increased in severe preeclampsia group,there was statistical significance between the difference （P 〈 0.05）.（3） The amount of microvessel density （MVD） in mild and severe preeclampsia group were 106 ±9,93 ±8,while 136 ±9 in control group,which were significantly reduced in mild and severe preeclampsia group,compared to tha

关 键 词：先兆子痫 眼蛋白质类 神经生长因子类 舍平类 胎盘 新生血管化 病理性 

分 类 号：R714.245[医药卫生—妇产科学]