麦洼牦牛H-FABP、HSL基因多态性及与生长性状的相关分析  

Association of Single Nucleotide Polymorphism of H-FABP and HSL Genes with Growth Traits in Maiwa Yak

作　　者：柴志欣[1] 王永[1] 罗晓林[2] 武志娟[1] 刘仲娜[1] 钟金城[1] 

机构地区：[1]西南民族大学动物遗传育种学国家民委-教育部重点实验室,成都610041 [2]四川省草原科学研究院,成都611731

出　　处：《中国农业科学》2013年第14期3022-3031,共10页Scientia Agricultura Sinica

基　　金：国家科技支撑计划项目(2012BAD13B06);西南民族大学研究生学位点建设项目(2011XWD S071007)

摘　　要：【目的】探讨心脏脂肪酸结合蛋白(heart fatty acid-binding protein,H-FABP)、激素敏感脂肪酶(hormone-sensitive lipase,HSL)基因在牦牛中的遗传多态性,进一步揭示牦牛各品系间的遗传分化以及候选基因与牦牛生长性状间的关联性,同时为2个候选基因在牦牛中的表达调控等研究提供理论依据,寻找可用于辅助选择的分子标记。【方法】采用PCR-SSCP技术和DNA测序技术对共100头麦洼牦牛个体的H-FABP、HSL基因的外显子部分进行遗传多态性分析,统计基因和基因型频率,进行Hardy-Weinberg平衡性检测,计算纯合度、杂合度、多态信息含量和有效等位基因数等遗传多态指标,分析候选基因不同基因型与体高、体重、体斜长、胸围、管围等生长性状的关联性。【结果】①麦洼牦牛H-FABP、HSL基因外显子部分均存在多态性,多态性检验均存在3种基因型;②适合性检验表明仅HSL基因外显子8上多态位点处于Hardy-Weinberg平衡状态,其余多态位点均偏离;③测序分析表明,H-FABP基因外显子4第7 339位(与原序列相比)发生单碱基突变(T→C),该突变属同义突变;HSL基因外显子7第6 883位发生G→C转换,以及第6816处发生碱基A→G突变,并导致编码氨基酸由天冬氨酸(D)转变为甘氨酸(G);外显子8第10 183 bp处发生A→G碱基突变,编码氨基酸由半胱氨酸(C)变为甘氨酸(G);④对各标记基因型生长发育指标(体高、体斜长、胸围、管围、体重)进行差异显著性检验,仅HSL基因外显子7上A6816G基因座差异极显著(P﹤0.01)。【结论】麦洼牦牛H-FABP、HSL基因存在遗传多态性,且HSL基因外显子7上A6816G基因座表现的多态有可能作为一种遗传标记。[ Objective ] Correlations between SNP of Maiwa yak heart fatty acid-binding protein （H-FABP）, hormone-sensitive lipase （HSL） genes and growth traits were studied in order to provide a theoretical basis for the two candidate genes in expression regulation in the yak. [Method]Maiwa yak and partial sequences of H-FABP and HSL genes were used as experimental samples and candidate genes. The genetic diversity of Maiwa yak was studied by PCR-SSCP, and the alleles frequency, genotype frequency, equilibrium analysis of Hardy-Weinberg, heterozygosity （H）, effective numbers of alleles （Ne） and polymorphism information content （PIC） by means of statistics method. The correlation between the candidate genes in different genotypes and the circumference of growth traits was analyzod.[ Result ]Polymorphism of H-FABP and HSL genes of some exon portions in Maiwa yak were found. There are three genotypes. The fitness test showed that only HSL gene exon 8 on polymorphic loci in Hardy-Weinberg equilibrium state and the rest of the polymorphic loci were deviation. H-FABP gene exon7 loci existed T---,C mutation of 7 339 bp which didn＇t result in change of amino acid. HSL gene exon7 loci existed in G---＊C mutation of 6 883 bp, A--＊G mutation of 6 816bpwhich led to amino acid from D to G. Analysis indicated that interaction effect of the polymorphism loci genotype and growth traits was significantly （P〈 0.01）. And A--＊G mutation of 10 183 bp in exon 8 which led to amino acid from C to G. [ Conclusion ] H-FABP and HSL genes in Maiwa yak have genetic polymorphism. A6816G genotype can be used as molecular marker.

关 键 词：麦洼牦牛 H-FABP基因 HSL基因 多态性 

分 类 号：S823.85[农业科学—畜牧学]