农杆菌介导的ZmHSD1基因转化玉米自交系  

Transformation of ZmHSD1 Gene into Maize Inbred Lines via Agrobacterium tumefaciens

作　　者：崔慧妮[1] 屠培培[1] 李晓丽[1] 李燕燕[1] 田凤龙[1] 张宪省[1] 孙庆泉[1] 

机构地区：[1]山东农业大学,山东泰安271018

出　　处：《山东农业科学》2013年第7期6-8,共3页Shandong Agricultural Sciences

基　　金：国家转基因生物新品种培育重大专项(2008ZX08003-003)资助

摘　　要：以NPTⅡ基因为筛选标记,利用农杆菌转化系统将目的基因ZmHSD1转入玉米自交系昌7-2、齐319和18-599的胚性愈伤组织,筛选抗性愈伤,并获得再生植株。对再生植株基因组DNA进行PCR检测表明,ZmHSD1基因已经整合到玉米基因组中,并最终获得转基因T1代种子。Using NPTⅡ gene as selective marker,ZmHSD1 gene was transformed into embryogenic calli of maize inbred lines Chang7-2,Q319 and 18-599 by Agrobacterium tumefaciens.Regenerated plantlets were obtained from resistant calli.The results of PCR detection of genomic DNA from regenerated plantlets showed that ZmHSD1 gene had been integrated into maize genome.And T1 transgenic seeds were obtained finally.

关 键 词：玉米 农杆菌 胚性愈伤组织 ZmHSD1基因 

分 类 号：Q785[生物学—分子生物学]