蜈蚣粗毒中多肽毒素组分生理活性初步分析  

Biochemical and Pharmacological Study of the Venom from The Centipede Scoropendra Subspinipes Mutilans Koch

作　　者：刘宇[1] 寻晓红[1] 

机构地区：[1]湖南理工学院化学化工学院,湖南岳阳414006

出　　处：《湖南理工学院学报（自然科学版）》2013年第2期53-56,66,共5页Journal of Hunan Institute of Science and Technology(Natural Sciences)

基　　金：国家自然科学基金项目(21172067);湖南省科技厅科技计划项目(2012SK3042);湖南省发展与改革委员会2011年科技基本建设项目

摘　　要：几乎所有蜈蚣都是肉食动物,都依靠其毒液来捕杀和消化猎物,蜈蚣粗毒为混合物,其主要成分为蛋白质.本文以蜈蚣粗毒为原料,通过反相高效液相色谱进行分离纯化,将纯化产物进行MALDI-TOF-MS鉴定,发现蜈蚣粗毒中具有多肽组分.收集多肽组分在DRG细胞上进行全细胞膜片钳电生理实验研究,发现其中4个组分具有抑制电压门控钠通道电流活性、4个组分具有抑制电压门控钾通道电流活性.表明蜈蚣毒素具有很好的开发应用前景,为后续工作打下了良好的实验基础.Almost all centipedes are predators and have venom glands. The primary purpose of centipede venoms is to kill or paralyze prey. Centipede venoms are complex chemical cocktails in which proteins are the main components. This study, investigated the biochemical and pharmacological properties of the venom of the centipede Scoropendra subspinipes mutilans Koch The RP-HPLC and MALDI-TOF MS spectra of the venom indicated that it contains diverse peptides with molecular weights from 3000-10000 Da. Using the whole-cell patch-clamp, it was found that the venom could inhibit both voltage-gated sodium channels （VGSC） and potassium channels （VGPC） in adult rat dorsal root ganglion （DRG） neurons. Furthermore, pharmacological activities of the 43 fractions collected from RP-HPLC were examined on both VGSCs and VGPCs in rat DRG neurons. Consequently, we identified four fractions containing peptide neurotoxins which could inhibit VGSCs, as well as the other four fractions that containing peptide neurotoxins which could inhibit VGPCs. This study indicates that the venom of the centipede Scoropendra subspinipes mutilans Koch is indeed a rich source of novel pharmacologically interesting peptides that would receive increased attention from pharmacologists and biochemists in the future.

关 键 词：蜈蚣毒素 反相高效液相色谱 MALDI TOF质谱 电压门控钠通道 电压门控钾通道 

分 类 号：Q501[生物学—生物化学]