黄芪甲苷对哮喘小鼠嗜酸性粒细胞浸润的影响及机制研究  

Study of astragaloside on infiltration of eosinophils in mouse model of allergic asthma

作　　者：蔡萃[1] 董竞成[2] 杜文静[2] 弓唯一[2] 刘宝君[2] 吴金峰[2] 曹玉雪[2] 

机构地区：[1]杭州市红十字会医院,杭州310003 [2]复旦大学附属华山医院,上海200032

出　　处：《中华中医药杂志》2013年第8期2278-2283,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划项目(973计划)(No.2009CB523001);国家自然科学基金项目(No.81173390);浙江省自然基金青年项目(No.LQ12H27005);上海卫生局中医药科研基金(No.2008LO18A);新教师基金课题(No.200802461053)~~

摘　　要：目的:观察黄芪甲苷对哮喘小鼠嗜酸性粒细胞浸润的影响,并初步探讨其机制。方法:用卵蛋白(OVA)致敏和激发建立哮喘小鼠模型,予黄芪甲苷腹腔注射后,观察小鼠行为学、肺组织病理学评分及肺泡灌洗液(BALF)中嗜酸性粒细胞计数的改变;用Luminex 200检测小鼠血清中RANTES、MCP-3及eotaxin 3种趋化因子及IL-3、IL-4、IL-5、IL-6、IL-10、GM-CSF、IFN-γ和TNF-α8种相关炎性细胞因子的水平;用RT-PCR观察小鼠肺组织中相关趋化因子受体CCR3、CCR5 mRNA表达的变化;用western blot检测小鼠肺组织中CCR3、CCR5及NF-κB p65蛋白的表达。结果:黄芪甲苷可使哮喘小鼠BALF中EOS的数量明显减少,改善哮喘小鼠的症状,减轻哮喘小鼠的肺组织炎症损伤;黄芪甲苷治疗后,哮喘小鼠血清中TNF-α、IFN-γ、IL-6、GM-CSF、IL-5等细胞因子及趋化因子eotaxin的浓度有下降的趋势,哮喘小鼠肺组织中CCR3 mRNA的表达下降,同时,还可抑制哮喘小鼠肺组织中CCR3和NF-κB p65蛋白的表达。结论:黄芪甲苷可抑制哮喘小鼠气道中嗜酸性粒细胞的浸润,此作用与其降低趋化因子、趋化因子受体的表达及相关炎性细胞因子的水平有关。Objective： To observe the effect of astragaloside 1V on infiltration of eosinophils in mouse model of allergic asthma, and to explore its possible mechanism. Methods： Using OVA sensitization and challenge to establish the mouse models of asthma and then treated intraperitoneally with astragaloside before each challenge. Observations of clinical symptoms, pulmonary histopathology and inflammatory scores. The levels of inflammatory cytokines and chemokines were measured by Luminex 200. Realtime-PCR and western blotting were also performed to analyze the effects of astragaloside on chemokine receptors expressions. The expressions of NF-κB p65 protein in the lung of mice were tested by western blotting. Results： Compared to the asthmatic model group, the clinical symptoms of mice treated with astragaloside had been relieved. And the extent of inflammation in the lung and the counts of eosinophil in BALF of mice treated with astragaloside were also reduced. The serum levels of cytokines and eotaxin in the mice of astragaloside groups had been decreased too. The expressions of CCR3 mRNA in the lung of mice treated with astragaloside had been significantly decreased （P〈0.05）, and the expressions of CCR3 and NF-κB p65 proteins had also been decreased. Conclusion： Astragaloside can inhibite the infiltration of eosinophils in the mice of allergic asthma. The effects of astragaloside on the expressions of chemokines, chemokine receptors and some cytokines are involved in this process. Key words： Astragaloside; Asthma; Eosinophil; Infiltration; Mechanism

关 键 词：黄芪甲苷 哮喘 嗜酸性粒细胞 浸润 机制 

分 类 号：R285[医药卫生—中药学]