大脑中动脉梗塞大鼠大脑中动脉蛋白激酶C表达及电针水沟穴干预的研究  

Study on expression of PKC in vascular smooth muscle of rats with middle cerebral artery occlusion and interfering effect of electro-acupuncture at Shuigou

作　　者：吕艳[1] 杜元灏[1] 徐彦龙[2] 高靓[1] 崔景军[3] 杨丽红[3] 何娇君[3] 李晶[1] 

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]甘肃省中医院,兰州730050 [3]天津中医药大学,天津300193

出　　处：《中华中医药杂志》2013年第8期2310-2314,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30973791);天津市应用基础及前沿技术研究计划(No.12JCZDJC25600)~~

摘　　要：目的:通过检测Wistar大鼠大脑中动脉梗塞(MCAO)后,与血管平滑肌收缩相关的蛋白激酶C(PKC)在大脑中动脉血管平滑肌细胞中不同时相的表达的水平,观察针刺水沟穴对PKC表达水平的干预效应,探讨PKC在MCAO后脑动脉平滑肌收缩中的作用,揭示针刺水沟穴调节急性脑梗死血管平滑肌收缩运动的分子机制及效应机制。方法:选用Wistar大鼠96只,随机分为模型组,针刺组,假手术组,每组30只,每组再分5个时相:0.5、1、3、6、12h,每时相6只,另设对照组6只。应用免疫组化法检测MCAO后PKC在大脑中动脉血管平滑肌不同时相的变化规律。结果:与对照组相比,模型组和针刺组各时相PKC表达上调(P<0.01)。假手术组各时相PKC表达水平与空白组相比无显著差异。在6h和12h,模型组与针刺组无显著性差异,其余时相针刺组表达均低于模型组(P<0.01)。模型组PKC表达水平随时相递增呈递增趋势(P<0.05),梗塞后第6小时达到高峰(P<0.05)。针刺组6h与12h之间无显著差异,其余时相之间有显著性差异(P<0.01)。结论:急性脑梗塞时期PKC蛋白表达上调,参与调节脑缺血状态下大脑中动脉平滑肌收缩,造成缺血性脑损伤;针刺水沟抑制MCAO模型大鼠大脑中动脉平滑肌PKC表达,对于缓解缺血后血管平滑肌痉挛具有重要作用。Objective： To observe the influence of electro-acupuncture （EA） at Shuigou （GV26） on vascular smooth muscle contraction related Protein Kinase C, to discuss the regulation effect of PKC on vascular smooth muscle contraction and to reveal the molecular mechanism of vascular smooth muscle contraction underlying the acupuncture at Shuigou （GV26） via using Wistar rat middle cerebral artery occlusion （MCAO） model （Middle Cerebral Artery Occlusion, MCAO）, detecting PKC expression in the brain artery vascular smooth muscle at different time. Methods： 96 healthy male Wistar rats were randomly divided into four groups： model group （n=30）, electro-acupuncture group （n=30）, sham-operation group （n=30）. Each group was further divided except control group into 0.5h, lh, 3h, 6h and 12h with 6 in each subgroup. Model group were set up by occlusion of the middle cerebral artery. Electro-acupuncture （15Hz, 20mA）~ was applied to ＇Shuigou＇ （GV 26） for 20 minutes. The middle cerebral arteries on the infarct side of the brain were fixed in formalin, embedded with paraffin, followed by sectioning and staining with immunohisiochemistry. Results： Compared with control group, PKC expression levels of model group and acupuncture group of each time-points were both increased （P〈0.05）. None of difference was observed between Sham-operation group and control group. Remarkable difference existed between model group and the electro-acupuncture at each time-point other than 6h and 12h （P〈0.05）. PKC expression level of model group was up-regulated with the increasing time-points （P〈0.05）. There was no difference in electro-acupuncture group between 6h and 12h other than the rest of the time-points （P〈0.05）. Conclusion： Up- regulation of PKC expression during cerebral ischemia underlying mechanisms of MCAO and ischemia injury; PKC expression in rat model of MCAO was significantly down-regulated by EA treatment.

关 键 词：蛋白激酶C 大脑中动脉梗塞 缺血性脑损伤 电针 水沟穴 

分 类 号：R245[医药卫生—针灸推拿学]