HPLC同时测定山银花中绿原酸和木犀草苷的含量  

Simultaneous Determination of chlorogenic acid and Luteolin-7-O-glucoside in Lonicerae Flos by HPLC

作　　者：李锦燊[1] 吴洪文[1] 

机构地区：[1]柳州市工人医院,柳州545005

出　　处：《北方药学》2013年第8期10-11,共2页Journal of North Pharmacy

基　　金：广西科技厅攻关基金资助项目(桂科攻11107009-2-4)

摘　　要：目的:建立同时测定山银花中绿原酸和木犀草苷含量的高效液相色谱法。方法:采用Phenomenex LunaC18柱(250mm×4.6mm,5μm)分离,以乙腈和0.4%醋酸溶液进行梯度洗脱,检测波长350nm。结果:绿原酸在77.15～771.5mg·L-1线性关系良好,平均回收率在101.7%(RSD1.71%);木犀草苷在1.536～15.36mg·L-1线性关系良好,平均回收率在101.6%(RSD2.03%)。结论:该方法简便,准确,快速易行,可同时测定山银花中绿原酸和木犀草苷的含量。Objective：To develop a HPLC method for simultaneous determining chlorogenic acid and Luteolin-7-O-glucoside in Lonicerae Flos.Methods：The analysis was carried out with Phenomenex Luna C18 column （250mm×4.6mm,5μm）.The mobile phase composed of acetonitrile-0.4% acetic acid with gradient elution,and the detecting wavelength was at 350nm.Results：Chlorogenic acid and Luteolin-7-O-glucoside were linear in the range of 77.15 ～771.5 and 1.536～15.36mg·L-1 .Their average recoveries were 101.7%（RSD1.71%）and 101.6%（RSD2.03%）respectively.Conclusion：The method is simple,accurate and quick,which can be used to control the quality of Lonicerae Flos.

关 键 词：山银花 高效液相色谱法 绿原酸 木犀草苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]