实时荧光定量PCR技术在转基因大豆A5547-127检测中的应用  

Application of real- time PCR technique in detection of genetically modified soybean A5547-127

作　　者：仇有文[1] 张明辉[1] 于艳波[1] 王傲雪[1] 高学军[1] 敖金霞[1] 袁肖寒[1] 刘营[1] 

机构地区：[1]东北农业大学生命科学与生物技术研究中心,哈尔滨150030

出　　处：《东北农业大学学报》2013年第7期6-10,F0002,共6页Journal of Northeast Agricultural University

基　　金：转基因生物新品种培育重大专项(2011ZX08004-002-002-004);哈尔滨市科技创新人才专项资金项目(2010RFQXN101)

摘　　要：采用实时荧光定量PCR技术检测耐除草剂转基因大豆A5547-127,并对检测方法的精确度和准确度指标进行评估,分析检测方法的特异性和适用性。结果表明,定量PCR检测方法的LOD和LOQ分别是1和10copies.μL-1,检测方法试验得到的所有偏差值均在允许范围之内,表明建立的定量PCR检测方法能够为转基因大豆A5547-127提供科学的定量检测依据,为转基因作物的鉴定、检测提供新方法。In this study, real-time PCR was used to detect herbicide tolerant genetically modified soybean A5547-127. The detection accuracy and accuracy indicators of this method were assessed, and the specificity of the detection method and applicability were analyzed. The results showed that the LOD and LOQ of this quantitative PCR method were 1 and 10 copies. μL-1, respectively. And all the deviations were within the permissible range. It indicated that this quantitative PCR detection method could provide scientific basis for genetically modified soybean A5547-127 quantitative detection, and also offered a new detection and identification method for GMOs.

关 键 词：大豆 实时荧光定量PCR A5547-127 定量检测 

分 类 号：S565.1[农业科学—作物学]