蔓越橘茎段离体培养  

Culture in vitro of cranberry stem

作　　者：张志东[1] 杨瑞芹[1] 李亚东[1] 刘海广[1] 吴林[1] 

机构地区：[1]吉林农业大学园艺学院,长春130118

出　　处：《东北农业大学学报》2013年第7期117-122,共6页Journal of Northeast Agricultural University

基　　金：农业部公益性行业(农业)科研专项(201103037;200903008-07-07);吉林省科技发展计划项目(20080713);吉林省财政厅项目(2012004)

摘　　要：研究适宜蔓越橘茎段离体培养的接种时期、消毒剂、培养基、ZT浓度及培养基pH。结果表明,2010年12月至2011年7月采集的茎段接种成活率达70%以上;采用12.5%的84消毒液处理5 min或0.1%HgCl2处理3 min的茎段成活率达70%以上;改良WPM培养基培养效果优于Anderson盐+MS有机物、MS、1/2MS和Anderson培养基;用改良WPM+ZT 1.0 mg·L-1初代培养的成活率为70.8%;用改良WPM+ZT 0.3 mg·L-1继代培养的株高、茎粗、增殖系数最高,节间数最多;最适培养基pH为4.5～5.0。The appropriate inoculation period, disinfectants, media, ZT concentration and pH value for cranberry stem culture in vitro were studied. The results showed that inoculation survival rate of stems collected from December 2010 to July 2011 reached above 70%. The survival rate of treatments with 12.5% 84-disinfection solution 5 min or 0.1% HgCI2 solution 3 min was above 70%. Culture effect with improved WPM was superior to that of Anderson solts＋MS organic material, MS, 1/2MS and Aderson mediums. Primary cultures survival rate of improved WPM＋ZT 1.0 mg- L1 treatments reached to 70.8%, treatment of improved WPM＋ZT 0.3 mg. L-1 during the subculture period got the highest plant height, stem diameter, multiplication coefficient and node number. The optimum pH of medium was 4.5-5.0.

关 键 词：蔓越橘 组织培养 茎段 

分 类 号：S565.1[农业科学—作物学]