甲基紫光度法测定红霉素肠溶片中的红霉素  

Spectrophotometry determination of erythromycin with methyl violet

作　　者：陈莲惠[1] 简易[2] 曹洪斌[1] 

机构地区：[1]川北医学院化学教研室,四川南充637000 [2]川北医学院物理教研室,四川南充637000

出　　处：《中国测试》2013年第4期58-60,共3页China Measurement & Test

基　　金：四川省教育厅重点基金项目(12ZA056);川北医学院重点培育基金项目(CBY11-A-ZP13)

摘　　要：在pH 6.8条件下,用二次石英蒸馏水为溶剂,甲基紫和红霉素在常温下可以反应形成稳定的离子缔合物,导致吸收光谱发生变化,且在一定范围内吸光度变化值与红霉素的浓度成正比,从而建立一种测定红霉素的分光光度法。实验表明:该方法灵敏度较高(ε=1.63×104L(/mol/cm)),精密度好(对15.00 mL 5.0×10-3mg/mL红霉素测定6次,RSD=0.9%)。红霉素的浓度在0.000 8～0.050 mg/mL范围内服从Beer定律,方法的检出限为0.18μg/mL。该方法简便可靠,重现性和选择性好,可用于红霉素肠溶片中红霉素含量的测定。In pH 6.8 acidic medium, methyl violet reacts with erythromycin in aqueous solution to form complex, which causes the absorption change of spectra, and A （absorbance, against the reagent blank） values are proportional to the concentration of erythromycin. The apparent molar absorptivity of the complex at 535 nm is 1.63×10^4L/（mol/cm）. Beer＇s law is obeyed in the range of 0.0008-0.050 mg/mL of erythomycin. Based on these, the authors developed a new spectropho tometric method for the determination of erythromycin. The determination limit of this method is found to be 0.18 μg/mL. The method is simple, reliable, repeatable, and it has good selectivity. The method can be applied to analyze the erythromycin content in enteric-coated tablets.

关 键 词：甲基紫 红霉素 分光光度法 

分 类 号：O657.32[理学—分析化学] TQ465.5[理学—化学]