不同地理来源芦笋茎枯病菌对杀菌剂抗药性的差异  

Difference of fungicide resistance of asparagus stem blight fungus from different geographic origins

作　　者：孟凡[1,2] 杨迎青[2] 兰波[2] 李湘民[2] 

机构地区：[1]江西农业大学农学院,南昌330045 [2]江西省农业科学院植物保护研究所,南昌330200

出　　处：《华中农业大学学报》2013年第5期61-65,共5页Journal of Huazhong Agricultural University

基　　金：公益性行业(农业)科研专项(201003074);国家重点基础研究发展计划(2011CB111603);江西省农业科学院创新基金(2011CBS006)

摘　　要：为明确不同省份芦笋茎枯病菌对内吸性和保护性杀菌剂抗药性的差异,采用菌丝生长速率法测定了6个省24个菌株对多菌灵和代森锰锌2种杀菌剂的抗药水平,并比较了其差异。结果表明:各省份菌株对2种杀菌剂的抗药性均存在明显差异,其中山东省菌株对多菌灵的抗药性最强,平均EC50值为1.10 mg/L,江西省和福建省菌株次之,平均EC50值分别为0.58 mg/L和0.33 mg/L;福建省菌株对代森锰锌的抗药性最强,平均EC50值为34.53 mg/L,河北省和山东省菌株次之,平均EC50值分别为15.88 mg/L和14.19 mg/L。多菌灵相对代森锰锌更易产生抗药性,其抗药菌株为7个,平均抗性水平为3.66,抗性频率为29.17%;而代森锰锌抗性菌株为4个,平均抗性水平为1.90,抗性频率为16.67%。24个菌株中,SD4对多菌灵和代森锰锌的抗药性均较强,抗性水平分别为10.44和3.01,表现出多抗性。To define the difference of the resistance level of asparagus stem blight fungus Phomopsis asparagi（Sacc.） Bubak from different provinces on systemic and protective fungicides,the resistance levels of 24 isolates from 6 provinces on carbendazim and mancozeb were determined by using the mycelial growth rate method.The isolates from Shandong Province expressed the most resistance on carbendazim with an average EC50 value of 1.10 mg/L,followed by the isolates from Jiangxi Province and Fujian Province,with an average EC50 values of 0.58 mg/L and 0.33 mg/L respectively.The isolates from Fujian Province expressed the most resistance on mancozeb with an average EC50 value of 34.53 mg/L,followed by the isolates from Hebei Province and Shandong Province,with average EC50 values of 15.88 mg/L and 14.19 mg/L respectively.Compared to mancozeb,it＇s easier for carbendazim to produce resistance,with 7 resistant isolates,3.66 as the resistant level and 29.17% as the resistant frequency,while 4 resistant isolates,with 1.90 as the resistant level and 16.67% as the resistant frequency,were included in mancozeb.Of 24 isolates,SD4 revealed resistance on both of carbendazim and mancozeb,with the resistance levels of 10.44 and 3.01 respectively,which expressed multiresistance.

关 键 词：芦笋茎枯病 天门冬拟茎点霉 地理来源 杀菌剂 抗药性 

分 类 号：S481.4[农业科学—农药学] S482.2[农业科学—植物保护]