ELISA与ICA对癌胚抗原检测的比较  

The Comparison of ELISA And ICA in Carcinoembryonic Antigen Detection

作　　者：彭文忠[1] 余高冰[1] 

机构地区：[1]深圳市宝安区西乡人民医院,广东深圳518102

出　　处：《中外医学研究》2013年第21期147-148,共2页CHINESE AND FOREIGN MEDICAL RESEARCH

摘　　要：目的:比较磁粒子捕获免疫发光法(ICA)和酶联免疫吸附分析法(ELISA)测定血癌胚抗原(CEA)的结果,了解这两种方法的优劣,探讨ICA和ELISA检测CEA的灵敏性和准确性。方法:ICA和ELISA作对比试验、回收试验和精密度试验。结果:对比试验,ICA法所测CEA含量为Y值,ELISA所测CEA含量为X值,则回归方程:Y=1.32X+8.23,相关系数r=0.991;回收试验,用ICA和ELSIA平均回收率分别为97.64%和96.73%;精密度试验,ELISA和ICA的批内精密度分别为11.74%和1.92%,批间精密度分别15.41%和3.68%。结论:ICA与ELISA线性相关性较好,批内、批间精密度均优于ELISA法,ICA的检测精度高,稳定性强,回收率高于ELISA,ICA法在检测准确度和稳定性上,较之ELISA具有很强的优越性。Objective： To compare magnetic particle capture immune luminescence（ICA） method and enzyme linked immunosorbent assay（ELISA） in determination of serum carcinoembryonic antigen （CEA） results, and understanding these two methods advantages and disadvantages and discuss ICA and ELISA detection of CEA sensitivity and accuracy.Methods： [CA and ELISA contrast test, recovery test and precision test.Results： Contrast test, Y was ICA method measured CEA content, X was ELISA measured CEA content, the regression equation： Y=1.32X＋8.23, correlation coefficient r=0.991.Recovery test, with the ICA and ELSIA average recovery rate was 97.64% and 96.73%.The accuracy test, ELISA and the group of ICA in precision were 11.74% and 1.92% respectively. Batch precision was between 15.41% and 3.68% respectively.Conclusion： ICA and ELISA linear correlation is better, in the group, and group were superior to that of ELISA method between precision, ICA has high accuracy, stability is strong, ICA recovery than ELISA is high, the ICA in detection accuracy and stability compared with ELISA has a strong advantage.

关 键 词：磁粒子捕获免疫发光 酶联免疫吸附分析 癌胚抗原 

分 类 号：R446.1[医药卫生—诊断学]