Xa21全生育期表达载体的构建  

Construction of Vector Expressing Xa21 during Whole Growth Stage

作　　者：曹玉鑫[1] 花龙[1] 赵天龙[1] 李明容[1] 夏志辉[1] 

机构地区：[1]海南大学农学院,海南海口570228

出　　处：《热带生物学报》2013年第2期133-137,145,共6页Journal of Tropical Biology

基　　金：国家自然科学基金(31071173);海南省教育厅高校科研资助项目(Hjkj2012-12);海南大学科研启动经费(KYQD1001)

摘　　要：利用PCR技术,以水稻品种明恢63基因组DNA与携Xa21完整基因组片段的载体pDBXa21作为模板,分别克隆了RSuS1启动子(RSP)、Xa21的开放阅读框至终止子的片段,并构建了RSP驱动水稻广谱抗白叶枯病基因Xa21的转基因表达载体。实时荧光定量PCR技术证实,水稻蔗糖合酶基因1(rice sucrose synthase gene 1,RSuS1)在水稻种子中低表达,在叶鞘与叶中全生育期高效表达。In this study,the results of realtime PCR of rice sucrose synthase gene 1（ RSuS1） showed that RSuS1 was highly expressed in sheath and leaf during whole growth stage. The promoter of RSuS1 was amplified from genomic DNA template of an indica rice variety＂Minghui 63＂,then the DNA fragment of Xa21 including open reading frame and terminator was amplified from vector pDBXa21 carrying completely genomic Xa21 fragment. In order to breed transgenic rice with resistance to bacterial leaf blight during whole growth stage and without risk of comestible safety,we constructed an expression vector that Xa21 was driven by RSP.

关 键 词：XA21 蔗糖合酶基因启动子 白叶枯病 水稻 全生育期 

分 类 号：Q812[生物学—生物工程]