角果木根系盐胁迫的防御机理  

Preliminary Study on the Salt Stress Defense Mechanisms of Ceriops tagal Root

作　　者：臧剑[1] 符秀梅[1] 王鹤鸣[1] 林道哲[1] 吴辉[1] 陈健妙[1] 陈银华[1,2] 

机构地区：[1]海南大学海南省热带生物资源可持续利用重点实验室,海南海口570228 [2]海南大学农学院,海南海口570228

出　　处：《热带生物学报》2013年第2期160-164,共5页Journal of Tropical Biology

基　　金：国家自然基金(31060040;31260345);教育部科学技术重点项目(207092)

摘　　要：以强耐盐性红树植物角果木为研究对象,采用不同浓度的NaCl溶液(0,150,300,450mmol·L-1)对角果木幼苗进行盐胁迫处理,测定角果木根部的SOD,POD,ASA,MDA等生理指标。结果表明:角果木在适应盐环境的过程中有新的蛋白产生,在盐胁迫早期(≤9h)MDA含量急剧上升,这种信号诱导了抗氧化防御系统的启动,使角果木根部抗氧化酶类及抗氧化物质均有所增加,说明角果木盐胁迫下通过启动抗氧化酶类、抗氧化物质以清除活性氧对根部的损伤,而后开始适应外界盐环境。Mangroves are woody plants that grow along tropical and subtropical coasts and form clumpy stands in intertidal zones. The anatomical and physiological features associated with their salt-tolerant lifestyles have not been well characterized,little is known about the impact of defensive enzymes on their adaptation to these saline environments. In this study,the enzyme activity of superoxide dismutase （ SOD） ,peroxidase （ POD） ,ascorbic acid（ ASA） and Malondialdehyde （ MDA） in the C. tagal roots treated by four concentrations （ 0,150,300,450 mmol·L^–1 ） of NaCl were studied in a greenhouse of Hainan University,Haikou,China. The results showed that the activity of MDA had a sharp increase at early stressed time,and this signal induces the appearance of antioxidative defensive system,including a certain increase of SOD,POD,ASA. which indicated that cell plasma membrane of C. tagal roots had rather strong resistance to the injury of salt ion. These results established a theoretical foundation for salt tolerance mechanism of C. tagal.

关 键 词：红树 角果木 盐胁迫 抗氧化系统 

分 类 号：Q945.78[生物学—植物学]