文心兰染色体的制片技术  

Preparation of Microscopic Slides of Oncidium for Chromosome Observation

作　　者：赵羿鸾[1,2] 黄琴[1,2] 贾贤[1,2] 王颖[2] 吴坤鑫[2] 陈雄庭[2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《热带生物学报》2013年第2期198-202,共5页Journal of Tropical Biology

摘　　要：以文心兰根尖为材料,采用常规制片法,对染色体制片的取样时间、预处理液、固定液、解离时间、染色液及染色时间进行了研究。结果表明:文心兰组织培养苗培养温度为25℃;适宜的取样时间为5～6月的上午10:00;材料放入1g·L-1的秋水仙素+0.002mol·L-1的8-羟基喹啉混合液中室温预处理4h的效果最好,用卡诺氏液固定12～24h,可获得较多的细胞分裂相,1mol·L-1的盐酸60℃水浴锅中恒温解离8min的效果较好,用改良的苯酚品红染色制片效果最好。The root tips of Oncidium flexuosum Lodd were used to study the effects of sampling time,pretreatment chemical solution,fixed solutions,hydrolyzing time and staining on the chromosome observation slides. The results showed that the optimal temperature for tissue culture of the Oncidium was at 25 ℃ and that the optimal sampling time of root tip was about 10： 00 a. m in May to June. The samples were pretreated with a solution of 0. 1% colchicine and 0. 002 mol·L^–1 8-hydroxyquinoline at room temperature for 4 hours,and fixed with the Carnoy＇s fixative for 24 hours,and then hydrolyzed with 1 mol·L^–1 HCl for 8 min at 60 ℃ and stained with modified Carbol fuchsin solution. The slides thus prepared for chromosome observation were proved to be optimum.

关 键 词：文心兰 根尖 染色体 制片技术 

分 类 号：Q343.2[生物学—遗传学]