长春市2009年手足口病来源的肠道病毒71型分子特征分析  

Molecular Characteristics of Enterovirus Type 71 from Hand Foot and Mouth Disease in Changchun City in 2009

作　　者：吴静[1] 孙宇[1] 周剑惠[2] 王爽[2] 刘红[1] 魏雷雷[2] 常新[2] 曹凤瑞[2] 田鑫[2] 闫莉[1] 

机构地区：[1]长春市疾病预防控制中心,吉林长春130033 [2]吉林省疾病预防控制中心,吉林省卫生厅病毒重点实验室,长春130062

出　　处：《中国疫苗和免疫》2013年第3期210-213,共4页Chinese Journal of Vaccines and Immunization

基　　金：国家科学技术艾滋病和病毒性肝炎等重大传染病防治专项传染病监测技术平台项目"辽宁及周边省市传染病病原谱流行规律研究"课题子课题"吉林省传染病病原谱流行规律研究"(编号:20012ZX10004209-003);吉林省人力资源与社会保障厅2010年度留学回国人员科学技术创新创业项目(2010-27);吉林省卫生厅2010年拔尖创新人才培养对象资助经费;吉林省卫生厅立项课题(编号:2010ZC025)

摘　　要：目的阐明长春市2009年手足口病(Hand Foot and Mouth Disease,HFMD)流行期间,肠道病毒71型(Enterovirus Type 71,EV71)分离株的VP1编码区基因特征。方法对从长春市2009年HFMD患者分离到的7株EV71分离株,采用逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)进行VP1编码区基因扩增,并对扩增产物进行序列测定,用生物信息学软件Bioedit和Mega 4.0进行同源性、亲缘关系分析。结果 7株EV71分离株的核苷酸和氨基酸序列同源性分别为96.5%～100%和98.6%～100%;与安徽省阜阳市2008年流行株的核苷酸和氨基酸序列同源性分别为97.1%～99.7%和98.6%～100%。7株分离株均属于C4a基因亚型,在基因亲缘关系树上处于两个相对独立的进化分支(较大进化分支Clade 1和较小进化分支Clade 2)。结论目前监测到的2009年致长春市HFMD流行的EV71为C4a基因亚型,并且至少有2个C4a基因亚型的传播链同时存在,其中Clade 1为优势传播链。Objective To clarify genetic characteristics of VP1 coding region of enterovirus type 71 （ EV71 ） during epidemic period of hand foot and mouth disease （ HFMD ） in Changchun prefecture in 2009.Methods EV 71 strains were isolated from the patients of HFMD during the epidemic in Changchun in 2009.The VP1 coding region of 7 EV71 strains was amplified by reverse transcription-polymerase chain reaction （ RT-PCR ） .The RT-PCR products were sequenced and then the sequences were analyzed for homology and phylogenetics by Bioedit and Mega4.0.Result The identity of nucleotide and amino acid of these 7 strains was 96.5 %-100 % and 98.6 %-100 % respectively;There are 97.1 %-99.7 % nucleotide identity and 98.6 %-100 % amino acid identity compared with those epidemic strains in Fuyang city of Anhui province in 2008.All 7 strains were C4a sub-genotype and they formed two relatively separated clusters （ one was major cluster and the other was smaller one ） .Conclusions All 7 EV71 strains causing HFMD epidemic were C4a sub-genotype.There are at least two transmission chains caused HFMD epidemic in Changchun in 2009 with clade1 being the dominant transmission chain.

关 键 词：手足口病 肠道病毒71型 分子特征 

分 类 号：R373.11[医药卫生—病原生物学]