“髓复康”对大鼠脑缺血损伤区和体外培养神经胶质瘢痕中硫酸软骨素蛋白多糖表达的抑制作用  

Inhibitory Effect of Traditional Chinese Medicine Suifukang on the Expression of Chondroitin Sulfate Proteoglycans in Cerebral Ischemia Zone of Rats and Glia Scar Cultured in Vitro

作　　者：张平 郑巧云 李国辉 

机构地区：[1]中医科学院望京医院,北京100102

出　　处：《中国药学杂志》2013年第15期1279-1283,共5页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(30701094)

摘　　要：目的研究益气活血补肾方"髓复康"对大鼠脑缺血损伤区和体外培养神经胶质瘢痕中硫酸软骨素蛋白多糖(CSPGs)表达的抑制作用。方法通过Koizumi法制作SD大鼠单侧大脑中动脉阻塞模型(MACO),将SD大鼠随机分为正常组、模型组、阳性对照组(激素组)、髓复康大剂量组、中剂量组和小剂量组,均在8,15和30 d 3个时间点取大鼠脑组织制作病理切片,通过免疫组化染色方法检测各组脑缺血损伤区硫酸软骨素蛋白多糖表达量的差异;建立体外培养神经胶质瘢痕模型,以血清药理学的方法选择不同浓度的含药血清,使用免疫组化染色方法分别在培养12,24,48 h后定量检测神经胶质细胞硫酸软骨素蛋白多糖的表达量。结果整体动物实验和体外培养的神经胶质细胞中,中药大剂量及中剂量组神经胶质细胞的反应性增生较轻,硫酸软骨素蛋白多糖表达最弱,均与模型组形成极显著差异(P<0.01),小剂量组的硫酸软骨素蛋白多糖表达也低于模型组,与之形成显著差异(P<0.05)。结论 "髓复康"可以抑制脑缺血损伤区硫酸软骨素蛋白多糖的表达,改善脑缺血损伤区轴突再生微环境,可能是其促进脑缺血损伤后轴突再生的机制之一。OBJECTIVE To study whether the traditional Chinese medicine Suifukang can inhibit the expression of chondroitin sulfate proteoglycans（CSPGs） in rats with middle cerebral artery occlusion. METHODS SD rats were selected and randomized into six groups： normal group, control group, methylprednisolone treatment group, and Suifukang high dose, middle dose and low dose treatment groups. Serum pharmacological method was used to prepare serum containing different concentrations of Suifukang. And glial scar model established in vitro was treated with the drug-eontaing serum. The expression of CSPGs was detected by immunohistology method. RESULTS The expression of CSPGs in the injured brain and glial scar in vitro were significantly inhibited by Suifukang （P 〈 0. 01 ）. CONCLUSION Suifukang can inhibit the expression of CSPGs. This function may be one of the mechanisms that Suifukang promotes the regeneration of axon and the functional recovery after ischemical injury of brain.

关 键 词：髓复康 脑缺血损伤 硫酸软骨素蛋白多糖 免疫组织化学 

分 类 号：R965[医药卫生—药理学]