四环素抗性基因在人工湿地中的去除及累积  

Removal and Accumulation of the Tetracycline Resistance Gene in Vertical Flow Constructed Wetland

作　　者：郑加玉[1] 刘琳[2] 高大文[1,3] 刘超翔[2] 

机构地区：[1]东北林业大学生态研究中心,哈尔滨150040 [2]中国科学院城市环境研究所,厦门361021 [3]哈尔滨工业大学市政环境工程学院,哈尔滨150090

出　　处：《环境科学》2013年第8期3102-3107,共6页Environmental Science

基　　金：教育部高等学校博士学科点专项科研基金项目(20100062110002);国家自然科学基金项目(51278480);福建省科技计划重点项目(2012Y0068)

摘　　要：以垂直潜流人工湿地系统为试验装置,考察了猪场养殖废水中四环素类抗生素抗性基因(tetW、tetM和tetO)在人工湿地中的去除及累积情况.结果表明,养猪废水中的3种抗性基因均有检出且含量较高,tetW、tetM和tetO的平均绝对含量分别为1.07×1010、4.03×1010和4.92×1010copies.L-1.通过湿地系统处理后,水体中抗生素抗性基因绝对拷贝数和相对表达量均明显降低,tetW、tetM和tetO的平均去除率分别为95.73%、92.21%和95.05%.在系统运行末期,湿地表层土壤和底层土壤中tetW、tetM和tetO的绝对拷贝数和相对表达量均有明显的升高,并且抗性基因在表层土壤中的累积水平高于底层土壤.因此,人工湿地系统可有效降低养猪废水中抗生素抗性基因的绝对及相对含量水平,系统的运行条件可对抗生素抗性基因在湿地系统中的积累产生影响.This paper investigated the efficiency and accumulation of vertical flow constructed wetland on removing tetracycline resistance（tet） genes（tetM,tetO,and tetW） from swine wastewater.The result indicated that all three tet genes were detected in raw wastewater,average absolute abundances of tetW,tetM,and tetO were 1.07×1010,4.03×1010 and 4.92×1010 gene copies per litre,respectively.Vertical flow constructed wetland could significantly reduce the content of wastewater antibiotics resistance genes,and average elimination rates were 95.73%,92.21% and 95.05%,respectively.Compare to the content of antibiotics resistance genes in unpolluted soil,the content of that in soil of the system had an obvious increase at the end stage of this study.Meanwhile,absolute abundances and relative abundances of three tet genes in surface layer of soil were higher than that in basement soil.The control condition and structure of construct wetlands would affect the accumulation of tetracycline resistance genes in the system.

关 键 词：人工湿地 养猪废水 抗生素抗性基因 实时荧光定量PCR 土壤 

分 类 号：X52[环境科学与工程—环境工程]