混料设计在优化橘小实蝇人工饲料中的应用  

Application of a Mixture Design to Optimize the Artificial Diet for the Oriental Fruit Fly, Bactrocera dorsalis

作　　者：陈毅超[1] 陈玲[1] 商晗武[1] 

机构地区：[1]中国计量学院生命科学学院/浙江省生物计量及检验检疫技术重点实验室,杭州310018

出　　处：《中国生物防治学报》2013年第3期354-361,共8页Chinese Journal of Biological Control

基　　金：浙江省重大农业科研专项(2006C12020);国家自然科学基金资助项目(30571241);浙江省重点科技创新团队项目(2010R50028)

摘　　要：用混料设计的方法研究了橘小实蝇人工饲料中蔗糖、酵母和麦麸的优化配比,建立了化蛹率、幼虫历期、蛹重、雌雄性比、单雌产卵量和卵孵化率6个评价指标的回归模型,并考察了原料间的交互作用,结果得到化蛹率的最大预期值为100%,幼虫历期的最小预期值为7.73 d,蛹重的最大预期值为17.76 mg,雌雄性比的理想预期值为1.00,单雌产卵量的最大预期值为381.22粒,卵孵化率的最大预期值为78.25%,除单雌产卵量外,各评估指标均存在交互作用,通过对6个指标条件的设定,最终得到优化配方2个,其三者配比分别为11.04%、3.27%、25.69%和12.11%、3.00%、24.89%,经验证结果理想,本研究表明混料设计方法对于昆虫人工饲料的开发应用具备可行性。Mixture design was employed to determine optimal proportions of the three diet ingredients, sugar, yeast and wheat bran, for the oriental fruit fly, Bactrocera dorsalis. Pupation rate, larval duration, pupal weight, sex ratio, oviposition, hatchability were correlated to diet ingredients to optimize the ingredient composition and the interaction among diet ingredients. We obtained the most predictable highest pupation rate of 100%, the shortest larval duration of 7.73 d, the biggest pupal weight of 17.76 mg, the most ideal sex ratio of 1.00, the highest fecundity of 381.22, the highest egghatching rate of 78.25%. With the exception of fecundity, there were interactive effects among the ingredients on the other parameters. Two optimal formulations of the ingredients, 11.04% sugar, 3.27% yeast and 25.69% wheat bran, and 12.11% sugar, 3.00% yeast and 24.89% wheat bran, were identified. The two formulations produced desired results. The mixture design method can be applied to develop or improve artificial diets of insects.

关 键 词：橘小实蝇 人工饲料 混料设计 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]