红火蚁高致病力黄绿绿僵菌菌株的筛选  

Selection of Metarhizium flavoviride Isolates to Control the Red Imported Fire Ant Solenopsis invicta

作　　者：许齐爱[1,2] 李佳颖[1,3] 任顺祥[1] 

机构地区：[1]教育部生物防治工程研究中心/华南农业大学昆虫学系,广州510640 [2]广东省江门市交通物业管理有限公司,江门529000 [3]湖南省衡阳出入境检验检疫局,衡阳421001

出　　处：《中国生物防治学报》2013年第3期384-388,共5页Chinese Journal of Biological Control

基　　金：国家"973"计划项目(2013CB127604)

摘　　要：从土壤中及昆虫寄主上分离获得了12株黄绿绿僵菌Metarhizium flavoviride菌株,在实验室条件下研究了不同菌株对红火蚁工蚁的致病力,并就筛选出的高致病力菌株SM076对红火蚁的控制效果进行了检测。试验结果表明,在相同孢子浓度下,红火蚁大型工蚁对菌株SM076的致死敏感性高于小型工蚁,两者处理后14 d的LC50分别为9.29×104和1.02×106孢子.mL 1。在1×107和1×108孢子.mL 1浓度的菌液处理下,红火蚁大型工蚁(兵蚁)和小型工蚁(工蚁)的LT50分别为7.88 d、9.11 d和6.14 d、7.81 d。随着黄绿绿僵菌孢子悬浮液浓度的增加,红火蚁的死亡率不断升高,致病时间缩短,致死中时间降低。研究表明,所筛选的黄绿绿僵菌菌株SM076是一株防控红火蚁危害的优良生防菌株。Twelve isolates of Metarhizium flavoviride from soil and insect hosts were evaluated for their pathogenicity to red imported fire ant Solenopsis invicta under laboratory conditions. The strain SM076 was more virulent to red imported fire ant S. invicta than other isolates. Results indicated that macrergate were more sensitive to SM076 than micrergate when they treated with same concentration of SM076. By immersion, LC50 values for old and micrergate on day 14 post-treatment were 9.29×104 and 1.02×106 conidia.mL-1. The LT50 values for macrergate and micrergate when treated with 1×107 and 1 ×108 conidia.mL-1 of SM076 suspension were 7.88, 9.11 d and 6.14, 7.81 d respectively. Moreover, the mortality orS. invicta increased and the lethal time reduced with the increase of the conidium concentration. Our research indicated that, the entomopathogenic fungi M. flavoviride strain SM076 could be the potential candidate in red imported fire ants control.

关 键 词：黄绿绿僵菌 红火蚁 致病力 生物防治 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]