HA纳米微粒对PEG-600低温保护剂玻璃化性质的影响  

Effects of HA nanoparticles on the vitrification of PEG-600 cryoprotectant solutions

作　　者：吕福扣[1] 刘宝林[1] 李维杰[1] 

机构地区：[1]上海理工大学生物热科学研究所,上海200093

出　　处：《低温与超导》2013年第8期1-4,13,共5页Cryogenics and Superconductivity

基　　金：国家自然科学基金(51076108);教育部新世纪优秀人才计划(07-0559);上海市东方学者计划资助课题

摘　　要：为了研究羟基磷灰石HA纳米微粒对低温保护剂玻璃化性质的影响,利用DSC测量了含有不同粒径(20nm,40nm,60nm)和不同质量浓度(0.1%,0.2%,0.4%,0.8%)HA纳米微粒的PEG-600(50%,w/w)溶液的玻璃化温度。试验结果表明:加入40nm,0.8%HA的PEG-600溶液的玻璃化转变温度最大,熔融温度最小,稳定性也最高。与未加纳米微粒的PEG-600溶液相比,玻璃化转变温度提高了5℃,熔融温度降低了4.5℃,稳定性提高了近30%。加入60nm,0.8%HA的PEG-600溶液的玻璃化转变温度和反玻璃化温度都是最小,而熔融温度最大,稳定性也最低。与未加纳米微粒的PEG-600溶液相比,反玻璃化温度降低了4.5℃,稳定性降低了14%。To study the effects of HA nanoparticles on the vitrification parameters of cryoprotectant solutions, the vitrification temperatures of PEG - 600 （50%, w/w） solutions with HA nanoparticles of different particle sizes （ 20nm, 40nm, 60nm） and dif- ferent concentrations （0.1% ,0.2% ,0.4% ,0.8 % ）were measured by differential scanning calorimeter （DSC）. The results indi- cated that the PEG- 600 solution containing 0.8% HA with the particle size of 40nm achieved the highest glass transition tem- perature, the lowest melting temperature, and the most stability. The glass transition temperature increased by 5℃, the melting temperature decreased by 4.5℃, and the stability of solutions increased by 30% when comparing with that of solution without nanoparticles. Experimental results showed that the PEG - 600 solution containing 0.8% HA with the particle size of 60nm had the highest melting temperature, the lowest glass transition temperature, the devitrification temperature, and the stability. The devitrification temperature decreased by 4.5℃, and the stability of solution decreased by 14% when comparing with that of solu- tion without nanoparticles.

关 键 词：纳米微粒 低温保护剂 玻璃化温度 DSC 

分 类 号：TB383[一般工业技术—材料科学与工程]