反转录环介导等温扩增技术在肿瘤学领域的应用  

Application of reverse transcription loop-mediated isothermal amplification technology in the field of oncology

作　　者：韩阳(综述)[1] 房居高(审校)[1] 倪鑫(审校)[2] 

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,100730 [2]首都医科大学附属北京儿童医院耳鼻咽喉头颈外科

出　　处：《肿瘤研究与临床》2013年第7期493-495,共3页Cancer Research and Clinic

基　　金：科技部国际科技合作基金（2012DFG31620）

摘　　要：反转录环介导等温扩增技术（RT-LAMP）是近年来新兴的一种核酸扩增技术。与聚合酶链反应（PCR）相比，RT-LAMP技术具有反应简单、快速、成本低廉、灵敏度高和特异性强等特点。虽然该技术已经成熟的用于病毒和其他病原体的基因检测，但其在肿瘤基因诊断方面的应用仍属新方法。文章旨在对RT-LAMP技术在肿瘤学领域的应用进行综述。Reverse transcription loop-mediated isothermal amplification（RT-LAMP）is a novel nucleic acid amplification technique which has been mainly used in a variety of viruses and other pathogens of gene detection. Compared with reverse transcriptase polymerase chain reaction（RT-PCR）, the RT-LAMP has advantages of reaction simplicity, rapidly, cost-effective, detection sensitivity and specificity. Nowadays, RT-LAMP technology is gradually used in various medical fields, but it is still a new method especially in cancer diagnosis of tumor gene. The article reviews the application of the RT-LAMP technology in the cancer area.

关 键 词：肿瘤 反转录环介导等温扩增技术 诊断 

分 类 号：R382.31[医药卫生—医学寄生虫学]