中国对虾幼体中肠腺的结构和细胞化学研究  

Study on structure and cytochemistry of midgut gland in larval Penaeus chinensis

作　　者：张志峰[1] 廖承义[1] 姜明[2] 刘晓云[2] 徐怀恕[1] 

机构地区：[1]青岛海洋大学海洋生命学院,山东青岛266003 [2]青岛海洋大学测试中心,山东青岛266003

出　　处：《中国水产科学》2000年第3期11-15,共5页Journal of Fishery Sciences of China

基　　金：联合国教科文组织资助项目!( UNESCO 861.359.8);国家攀登计划B资助项目!(PDB6-2-2)

摘　　要：用组织学、细胞学以及细胞化学等手段对中国对虾中肠腺的结构发生和功能进行研究。结果表明，中肠腺始于Ｎ４～５期，为中肠前端先后向两侧突出的２对盲囊，第１对中肠腺育囊至Ｍ３期退化消失，成体中肠腺由第２对中肠腺盲囊发育而成。中肠腺小管上皮在Ｚ２期细胞已有了分化，由４类细胞组成，即胚细胞（Ｅ细胞）、纤维细胞（Ｆ细胞）、泡状细胞（Ｂ细胞）和吸收细胞（Ｒ细胞）。电镜下，腺上皮细胞在发育的各个时期基本一致；Ｅ细胞的核质比大，具有很强的分裂能力；Ｆ细胞舍丰富的粗面内质网及酶原颗粒，该细胞在微绒毛处可见ＡＣＰ活性，主要进行细胞外消化；Ｂ细胞有明显地微胞饮现象，该细胞内可见明显的ＡＣＰ阳性反应物质，表现出旺盛的细胞内消化活动；Ｒ细胞线粒体和滑面内质网丰富，其内贮存有脂肪颗粒和糖原。Genesis and function through histo and cytology and cyto-chemistry are researched in midgut gland of Penaeus chinensis at larval stage. The results show that 2 pairs of midgut gland diverticula protrude from the midgut at N4--5, of which the first one degenerates gradually and disappears at M3, while the second one would develop into the midgut gland in adult. The midgut gland cell differentiation occurs at Z2. There are 4 types of cells, i. e., embryonic cell (E-cell), fibrillar cell (F-cell), blister cell (B-cell) and resorptive cell(R-cell). The epithelial cells of gland show no difference during larval development. The proportion of nucleus and cytoplasm in E-cell is high, but organelles are not rich. E-cell has obvious ability of division. In F-cells plenty of rough endoplasmic reticulum and zymogen can be observed. And in F-cell there is ACP activity at microvilli obviousely. On the top of B-cell, pinocytotic phenomenon can be observed distinctly, and there exist some small vesicles and large digestive ones. Because of different physiological states in the cells, ACP activities occur at different sites obviously. The early R-cells are very rich in mitochondria and smooth endoplasmic reticulum with plenty of fatty granular and glycogen.

关 键 词：中国对虾 幼体 中肠腺 细胞学 细胞化学 

分 类 号：Q959.223.5[生物学—动物学]