在体研究前扣带回皮层对伤害性热刺激的反应  

Anterior cingulate cortex neuronal response to noxious thermal stimulation underling in vivo path-clamp

作　　者：马丽清[1] 宁丽[1] 王英伟[1] 

机构地区：[1]上海交通大学医学院附属新华医院麻醉与重症医学科,200092

出　　处：《临床麻醉学杂志》2013年第7期693-695,共3页Journal of Clinical Anesthesiology

基　　金：国家自然科学基金(项目编号:81171169)

摘　　要：目的采用在体穿孔全细胞膜片钳技术,记录麻醉大鼠的前扣带回皮层(ACC)神经元对热刺激的反应,以证实ACC参与热感觉信息的处理。方法成年雄性SD大鼠10只,腹腔注射乌拉坦麻醉后,将大鼠头部固定于立体定位仪上进行在体穿孔膜片钳记录,分别给予大鼠后爪37、60℃的热刺激,记录ACC神经元对两种热刺激的反应。结果麻醉状态下,给予大鼠后爪伤害性热刺激(60℃)后,ACC神经元膜电位活动由低频转变为高频,分布由双峰转变为单峰,平均膜电位上升至(-53.62±6.38)mV,明显高于无刺激时和非伤害性刺激(37℃)时(P<0.01),且平均膜电位绝对值增加(5.43±2.87)mV。非伤害性热刺激(37℃)不能引起ACC电活动变化。结论 ACC参与伤害性热感觉信息的处理。Objective To confirm that anterior cingulate cortex （ACC） is involved in processing thermal information. We carried out in vivo perforated whole-cell patch-clamp to record rat ACC neuronal response to thermal stimulation under anesthesia. Methods Ten adult male Sprague- Dawley rats were injected with urethane intraperitoneally. The head of rat was fixed with a stereotaxic apparatus. In vivo perforated whole-cell patch-clamp was used to record the activity of ACC neurons. Two kinds of thermal stimulation （37℃ and 60 ℃ ） were given to the hind-paws of anaesthetic rats, meanwhile we recorded the neuronal responses evoked by thermal stimulation. Results Under anesthesia, after given the noxious thermal stimulation （60 ℃）, the ACC neuronal activity became faster,and distributed from bilaterally to unimodally, the average （MP） raised to （-53.62±6.38） mV, significantly higher than those without stimulation or under innocuous thermal stimulation （P〈 0. 01）,and the absolute value of MP increased （5.43 ± 2.87） inV. While the innocuous thermal stimulation （37 ℃ ） could not evoke such response. Conclusion ACC is involved in processing noxious thermal information.

关 键 词：前扣带回皮层 伤害性热刺激 在体穿孔全细胞膜片钳 

分 类 号：R614[医药卫生—麻醉学]