河南省新确证艾滋病感染者毒株亚型分析  

Genetic subtype HIV among newly ascertained infection cases in Henan province

作　　者：孙国清[1] 刘佳[1] 王哲[1] 崔为国[1] 

机构地区：[1]河南省疾病预防控制中心性病艾滋病防治研究所,郑州450016

出　　处：《中国公共卫生》2013年第8期1104-1107,共4页Chinese Journal of Public Health

基　　金：国家"十一五"重大科技专项(2009ZX10001-017)

摘　　要：目的了解河南省新确证的艾滋病感染者人类免疫缺陷病毒(HIV)亚型分布情况及其流行特征。方法随机采集351例河南省2010年新确证HIV-1阳性者的全血样本,分离血浆,提取RNA,PCR扩增gag区基因并进行序列测定,对测序得到的序列进行生物信息学分析,确定基因亚型。结果最终得到210条gag区基因序列,包含B、CRF01-AE、CRF07-BC、CRF08-BC、CRF01A1、CRF01B和CRF31-BC 7种亚型,其中B亚型185例(88.09%),CRF01-AE亚型12例(5.71%),CRF07-BC亚型8例(3.81%),CRF08-BC亚型2例(0.95%),CRF01A1、CRF01B和CRF31-BC各1例(0.48%)。结论河南省新确证的艾滋病感染者艾滋病毒有7种亚型,B亚型仍然占主导地位。Objective To investigate genetic subtype distribution and sequence characteristics of human immunodeficiency virus type 1（HIV-1）strains prevalent in Henan province during 2010. Methods Three hundred and fifty one blood samples were collected from newly ascertained HIV-1 infection individuals in Henan during 2010 and the plasma specimens were seperated from the samples.RNAs were extracted and the complete gag gene was amplified by reverse transcription（RT）-PCR and nested-PCR.The PCR products were sequenced.All sequences were analysed with Bioinformatics software. Results Among the 351 HIV-1 plasma specimens,210 gag HIV-1 gene fragments were amplified and analyzed.Seven HIV-1 subtypes or circulating recombinant forms（CRF）including B,CRF01-AE,CRF07-BC,CRF08-BC,CRF01A1,CRF01B,and CRF31-BC were identified.Genotyping showed that the number of subtype HIV-1 was 185（88.09%）for B,12（5.71%）for CREDI-AE,8（3.81%）for CRFO7-BC,2（0.95%）for CRF08-BC,and 1（0.48%）for CREOIAI,CRFOIB and CRF31-BC,respectively. Conclusion There are seven HIV-1 genetic subtypes pevalent in Henan province and subtype B is the main subtype.

关 键 词：艾滋病 巢式聚合酶链反应 序列测定 亚型 

分 类 号：R183.7[医药卫生—流行病学]