壬基酚对大鼠前列腺细胞凋亡影响及机制  

Effects of nonylphenol on prostate cell apoptosis and expressions of caspase-3,caspase-8,and caspase-9 mRNA in male Wistar rats

作　　者：朱建林[1] 陈昱[1] 刘瑾[1] 杨华[1] 吴传城[1] 李昱辰[1] 陈锦[1] 任南[1] 

机构地区：[1]福建医科大学公共卫生学院职业与环境卫生学系,福建福州350004

出　　处：《中国公共卫生》2013年第8期1187-1189,共3页Chinese Journal of Public Health

基　　金：福建省自然科学基金(2009J01150)

摘　　要：目的探讨壬基酚对大鼠前列腺组织细胞凋亡及caspase-3、caspase-8和caspase-9 mRNA表达影响。方法健康清洁级Wistar雄性大鼠50只,按体重随机分为5组,分别为12.5、25、50、100 mg/kg壬基酚染毒组和对照组(橄榄油),每组10只;皮下注射染毒(1 mL/kg),每天1次,连续染毒30 d;Tunel法观察前列腺前叶组织细胞凋亡,实时荧光定量聚合酶链式反应(RT-PCR)法观察组织中caspase-3、caspase-8、caspase-9 mRNA表达。结果 12.5、25、50、100 mg/kg壬基酚染毒组大鼠前列腺细胞凋亡率分别为(3.07±1.31)%、(5.21±1.47)%、(6.31±1.29)%、(8.90±0.18)%,均低于对照组的(12.73±2.19)%(P<0.05);与对照组比较,12.5、25、50、100 mg/kg壬基酚染毒组大鼠前列腺组织细胞caspase-3、caspase-8、caspase-9 mRNA表达均降低(P<0.05)。结论壬基酚可致雄性大鼠前列腺细胞凋亡率下降,其机制可能与前列腺细胞caspase-3、caspase-8和caspase-9 mRNA表达降低有关。Objective To evaluate effects of nonylphenol（NP）on rat prostate tissue apoptosis and expressions of caspase-3,caspase-8,and caspase-9 mRNA. Methods Fifty healthy Wistar rats were randomly divided into five groups（one olive control group and 4 groups exposed to NP at the doses of 12.5,25,50,and 100 mg/kg）based on body weight.The exposure groups were admimistered 1 mg/kg of NP hypodermic injection once a day for 30 days.The prostate tissue apoptosis was observed with teminal transferase-mediated biotin dUTP nick end labeling（TUNEL）and expressions of caspase-3,caspase-8,and caspase-9mRNA in prostate tissue were detected with real-time reverse transcription PCR（RT-PCR）. Results The prostate apoptosis rate in 12.5,25,50,and 100 mg/kg NP group were 3.07±1.31%,5.21±1.47%,6.31±1.29%,and 8.90±0.18%,all lower than that in the control group（12.73±2.19%）（P〈0.05 for all）.The expressions of caspase-3 mRNA,caspase-8 mRNA,and caspase-9 mRNA in all NP exposed groups were lower than those in the control group（P〈0.05 for all）. Conclusion NP decreases the apoptosis of prostate tissue in male Wistar rats,which may be one of the mechanisms for prostate hyperplasia in rats caused by NP.

关 键 词：环境内分泌干扰物 壬基酚 大鼠 凋亡基因 前列腺增生 

分 类 号：R994.6[医药卫生—毒理学]