洗涤血小板各组分促人/鼠成纤维细胞增殖初探  

Preliminary study of human washed platelet to promote human and mice cell proliferation

作　　者：陈洁[1] 王红[1] 钟锐[1] 贺曾[1] 吴瑕[1] 刘嘉馨[1] 

机构地区：[1]中国医学科学院 北京协和医学院 输血研究所,四川成都610052

出　　处：《中国输血杂志》2013年第7期607-611,共5页Chinese Journal of Blood Transfusion

摘　　要：目的分析人血小板及其洗涤后获得的各组分补体灭活前后对小鼠成纤维细胞(L929)和人成纤维细胞(HDP)的增殖作用。方法将所制备的新鲜人富血小板血浆(PRP)3份及血小板浓缩液(PC)5份(50 mL/份),分别用20 mL 0.9%生理盐水洗涤2次,重悬以获得贫血小板血浆(PPP)、生理盐水上清、洗涤血小板(WP)3个组分,同未经处理的PRP及PC一道,分别冻融留取对照样品后作补体灭活处理,ELASA方法测试补体灭活前后血小板源性生长因子(PDGF-AB)、转化生长因子(TGF-β1)、血管内皮生长因子(VEGF)、表皮生长因子(EGF)含量,CCK-8试剂盒测定补体灭活前后对L929/HDP细胞的增殖作用。结果 PRP及洗涤各组分补体灭活前后PDGF-AB、VEGF及EGF含量比较,P>0.05,PC及各组分补体灭活前后PDGF-AB、VEGF、EG及TGF-β1含量比较均无明显差异(P>0.05)。PRP及WP促L929细胞增殖比较:PRP为0.68±0.12 vs 2.13±0.18(P<0.05),WP为0.69±0.12 vs 0.66±0.13(P>0.05);PC及WP促L929细胞增殖比较:PC为0.42±0.05 vs 1.94±0.19(P<0.05),WP为1.69±0.13 vs 1.93±0.19(P>0.05)。补体灭活前后,PRP及WP促HDP细胞增殖:PRP为1.36±0.09 vs1.30±0.11(P>0.05),WP为1.26±0.04 vs 1.33±0.10(P>0.05);PC洗涤组分促细胞增殖:PC为1.32±0.07 vs1.28±0.09(P>0.05),WP为1.39±0.13 vs 1.44±0.13(P>0.05)。结论补体灭活对PRP与PC及洗涤获得的各组分PGFs含量影响较小,PGFs含量与促L929/HDP细胞的增殖作用存在非线性正相关关系,PGFs促细胞增殖可能存在种间差异。Objective To investigate the influences of human washed platelet components in mice and human fibroblast proliferation before and after complement inactivated. Methods Three doses（50 mL/dose）of fresh platelet rich plasma（PRP） and 5 doses of platelet concentrate （PC） were washed twice with 20 mL normal saline, then suspended to get platelet poor plasma（PPP） ,physiological saline supernatant, and washed platelet （WP） ,and meanwhile untreated PRP and PC was prepared to obtain platelet growth factors through Freeze-thaw method. The components of all samples before and af- ter inactivation were tested platelet-derived growth factor AB（PDGF-AB） ,transforming growth factor-beta 1 （ TGF-[31 ） ,vascular endothelial growth factor （VEGF） , and epidermal growth factor （EGF） contents by ELASA, and the promoting human and mice cell proliferation with CCK - 8 kit. Results For PRP and its washed components, there was no significant difference in the contents of PDGF-AB, VEGF and EGF before and after the complement inactivation （ P 〉 0. 05 ）. For the contents of PDGF-AB, VEGF,EG and TGF-β1 in PC and its washed components ,there was also no statistical difference （P 〉 0.05）. For Promoting L929 Proliferation, before and after the complement inactivation,PRP was 0. 68± 0. 12 vs 2. 13 ± 0. 18（P 〈0. 05） and its WP was 0. 69 ±0. 12 vs O. 66 ±0. 13（P 〉0. 05） ;PC was 0. 42 ±0. 05 vs 1.94 ±0. 19（P 〈0. 05） and its WP was 1.69 ±0. 13 vs 1.93 ±0. 19 （P 〉0.05）. For promoting HDP proliferation,before and after the complement inactivation, PRP was 1.36 ± 0.09 vs 1.30 ± 0. 11 （ P 〉 0. 05 ） and its WP was 1.26 ± 0. 04 vs 1.33 ± 0. 10 （ P 〉 0. 05 ） ; PC was 1.32±0.07 vs 1.28 ±0.09 （P 〉0.05） and its WP was 1.39 ±0. 13 vs 1.44 ±0. 13（P 〉0.05）. It Suggested that PRP and PC groups had no statistical differences in promoting HDP cell proliferate before and after, complement inactivated. Conclusion Complement inactivated processhas a small in

关 键 词：人洗涤血小板 补体灭活 血小板生长因子 细胞增殖 成纤维细胞 小鼠 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R446.112[医药卫生—基础医学]