LC-MS/MS法测定人血浆中地高辛的浓度  

Determination of Digoxin Concentration in Human Plasma by LC-MS/MS

作　　者：张萌萌[1] 唐云彪[1] 隋因[1] 颜鸣[1] 崔红霞[1] 史国兵[1] 

机构地区：[1]沈阳军区总医院药剂科,沈阳110016

出　　处：《中国药房》2013年第30期2835-2837,共3页China Pharmacy

摘　　要：目的:建立测定人血浆中地高辛浓度的方法,用于临床地高辛血药浓度监测。方法:血样以乙醚-二氯甲烷(3∶2)处理后,采用液-质联用(LC-MS/MS)法进样测定,色谱柱为Ultimate XB-C18,流动相为5mmol/L乙酸铵(0.1%甲酸)水溶液-乙腈(梯度洗脱),流速为0.3ml/min,内标为尼菲卡兰。结果:地高辛血药浓度在0.1～10μg/L范围内线性关系良好,最低定量限为0.1μg/L;日内、日间相对标准偏差(RSD)<15%;提取回收率为75.2%～82.5%。结论:本文所建立的方法操作简单、灵敏、快速,适用于地高辛临床常规血药浓度的监测。OBJECTIVE：To establish a method for the determination of digoxin concentration in human plasma,and to monitor the plasma concentration of digoxin in the clinic.METHODS：After treated with aether-dichloromethane （3∶2）,blood sample was determined by LC-MS/MS.The separation was achieved on Ultimate XB-C18 column with mobile phase consisted of 5 mmol/L ammonium acetate （0.1% formic acid）-acetonitrile （gradient elution） at the flow rate of 0.3 ml/min.Nifekalant was used as internal standard.RESULTS：The linear ranges of digoxin were 0.1-10 μg/L,and the lowest limit of quantification was 0.1 μg/L.The intra-day and inter-day RSDs were less than 15%,respectively.The extraction recoveries were 75.2%-82.5%.CONCLUSIONS：The method is simple,sensitive,rapid and suitable for plasma concentration monitoring of digoxin.

关 键 词：地高辛 液-质联用法 血药浓度 药动学 

分 类 号：R969.1[医药卫生—药理学] R972[医药卫生—药学]